Kondo N, Shinoda S, Agata H, Nishida T, Miwa Y, Fujii H, Orii T
Department of Pediatrics, Gifu University School of Medicine, Japan.
Biotherapy. 1992;5(4):281-4. doi: 10.1007/BF02179045.
Scores of radioallergosorbent test (RAST) for cow's milk or buckwheat flour and proliferative responses of peripheral blood mononuclear cells (PBMCs) to bovine serum albumin and beta-lactoglobulin or buckwheat flour were measured in cow's milk or buckwheat flour sensitive patients with allergic tension-fatigue syndrome. In all 3 cow's milk sensitive patients with allergic tension-fatigue syndrome, RAST scores for cow's milk were negative or slightly positive, but PBMCs well responded to bovine serum albumin and beta-lactoglobulin, but not to ovalbumin. In a buckwheat flour sensitive patient with allergic tension-fatigue syndrome, RAST scores for buckwheat flour were negative, but PBMCs well responded to buckwheat flour, but not to ovalbumin, bovine serum albumin and beta-lactoglobulin. Conversely, in cow's milk or buckwheat flour sensitive patients with immediate allergic symptoms, RAST scores for offending foods were positive although PBMCs did not respond to offending food antigens. These results suggest that proliferative responses of PBMCs to food antigens are very useful for detection of offending foods in allergic tension-fatigue syndrome.
在患有过敏性紧张性疲劳综合征的牛奶或荞麦面粉敏感患者中，测量了牛奶或荞麦面粉的放射变应原吸附试验（RAST）评分以及外周血单核细胞（PBMC）对牛血清白蛋白和β-乳球蛋白或荞麦面粉的增殖反应。在所有3例患有过敏性紧张性疲劳综合征的牛奶敏感患者中，牛奶的RAST评分呈阴性或弱阳性，但PBMC对牛血清白蛋白和β-乳球蛋白反应良好，而对卵清蛋白无反应。在1例患有过敏性紧张性疲劳综合征的荞麦面粉敏感患者中，荞麦面粉的RAST评分呈阴性，但PBMC对荞麦面粉反应良好，而对卵清蛋白、牛血清白蛋白和β-乳球蛋白无反应。相反，在有速发型过敏症状的牛奶或荞麦面粉敏感患者中，致敏食物的RAST评分呈阳性，尽管PBMC对致敏食物抗原无反应。这些结果表明，PBMC对食物抗原的增殖反应对于检测过敏性紧张性疲劳综合征中的致敏食物非常有用。
