Kondo N, Agata H, Fukutomi O, Motoyoshi F, Orii T
Department of Pediatrics, Gifu University School of Medicine, Tsukasa, Japan.
J Allergy Clin Immunol. 1990 Aug;86(2):253-60. doi: 10.1016/s0091-6749(05)80073-9.
The proliferative responses of peripheral blood mononuclear cells (PBMCs) to ovalbumin or bovine serum albumin in children with atopic dermatitis (AD) who are sensitive to hen's egg or cow's milk were significantly higher than responses of PBMCs of healthy children and hen's egg- or cow's milk-sensitive children with immediate symptoms. However, the percentages of positive RAST for hen's egg or cow's milk in the patients with AD were lower than percentages in the patients with immediate symptoms. In the patients with AD, there were no significant correlations between the proliferative responses of PBMCs and the RAST values. There were no significant differences of RAST scores among groups of patients having different degrees of severity of AD. The proliferative responses of PBMCs to ovalbumin or bovine serum albumin in patients with severe AD or moderate AD who were sensitive to hen's egg or cow's milk tended to be higher than responses of patients with mild AD, respectively, but there were no significant differences in those results. Taken together, the combination of RAST and the detection of proliferative responses of PBMCs to each food antigen is very useful in the diagnosis of hypersensitivity in children with AD who are sensitive to food allergens.
对鸡蛋或牛奶过敏的特应性皮炎（AD）患儿外周血单核细胞（PBMCs）对卵清蛋白或牛血清白蛋白的增殖反应，显著高于健康儿童以及有速发症状的鸡蛋或牛奶过敏儿童的PBMCs反应。然而，AD患者中对鸡蛋或牛奶的RAST阳性率低于有速发症状的患者。在AD患者中，PBMCs的增殖反应与RAST值之间无显著相关性。不同AD严重程度组患者的RAST评分无显著差异。对鸡蛋或牛奶过敏的重度AD或中度AD患者的PBMCs对卵清蛋白或牛血清白蛋白的增殖反应，分别倾向于高于轻度AD患者，但这些结果无显著差异。综上所述，RAST与检测PBMCs对每种食物抗原的增殖反应相结合，对诊断对食物过敏原过敏的AD患儿的超敏反应非常有用。
