大肠杆菌中“延伸 -10”启动子的鉴定与分析
Identification and analysis of 'extended -10' promoters in Escherichia coli.
作者信息
Mitchell Jennie E, Zheng Dongling, Busby Stephen J W, Minchin Stephen D
机构信息
School of Biosciences, The University of Birmingham, Edgbaston, Birmingham B15 2TT, UK.
出版信息
Nucleic Acids Res. 2003 Aug 15;31(16):4689-95. doi: 10.1093/nar/gkg694.
Abstract
We have compiled and aligned the DNA sequences of 554 promoter regions from Escherichia coli and analysed the alignment for sequence similarities. We have focused on the similarities and differences between promoters that either do or do not contain an extended -10 element. The distribution of -10 and -35 hexamer element sequences, the range of spacer lengths between these elements and the frequencies of occurrence of different nucleotides, dinucleotides and trinucleotides were investigated. Extended -10 promoters, which contain a 5'-TG-3' element, tend to have longer spacer lengths than promoters that do not. They also tend to show fewer matches to the consensus -35 hexamer element and contain short runs of T residues in the spacer region. We have shown experimentally that the extended -10 5'-TG-3' motif contributes to promoter activity at seven different promoters. The importance of the motif at different promoters is dependent on the sequence of other promoter elements.
摘要
我们已汇编并比对了来自大肠杆菌的554个启动子区域的DNA序列，并分析了比对结果中的序列相似性。我们重点研究了含有或不含有扩展 -10元件的启动子之间的异同。研究了 -10和 -35六聚体元件序列的分布、这些元件之间的间隔长度范围以及不同核苷酸、二核苷酸和三核苷酸的出现频率。含有5'-TG-3'元件的扩展 -10启动子往往比不含该元件的启动子具有更长的间隔长度。它们与共有 -35六聚体元件的匹配也往往较少，并且在间隔区域含有短的T残基序列。我们通过实验表明，扩展 -10的5'-TG-3'基序有助于七个不同启动子的启动子活性。该基序在不同启动子中的重要性取决于其他启动子元件的序列。
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本文引用的文献
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