Trommer H, Plätzer M, Wolf R, Neubert R H H
Institute of Pharmaceutics and Biopharmaceutics, School of Pharmacy, Martin Luther University Halle-Wittenberg, Germany.
Skin Pharmacol Appl Skin Physiol. 2003 Sep-Oct;16(5):291-304. doi: 10.1159/000072069.
Lipid model systems consisting of the major components of the stratum corneum intercellular lipid matrix were studied to investigate the ultraviolet-radiation-mediated damage of these biomolecules. Pure lipids and liposomes were irradiated using a lamp emitting a solar radiation spectrum. The influences of the irradiation and the effects of added iron ions were studied by electrospray ionization mass spectrometry (MS) with an ion trap analyser. Exact mass measurements were carried out using a time-of-flight mass spectrometer. Only linolenic acid and cholesterol were found to be subject to oxidative changes caused by UV irradiation whereas the other lipids examined (dipalmitoylphosphatidylcholine, ceramide III and cholesterol sulphate) were stable to oxidative stress. Several lipid adducts were observed upon analysis of the liposomes. The composition of these adducts was identified by MS/MS experiments.
研究了由角质层细胞间脂质基质的主要成分组成的脂质模型系统，以研究紫外线辐射介导的这些生物分子的损伤。使用发射太阳辐射光谱的灯照射纯脂质和脂质体。采用带有离子阱分析仪的电喷雾电离质谱（MS）研究了辐照的影响以及添加铁离子的作用。使用飞行时间质谱仪进行精确质量测量。结果发现，只有亚麻酸和胆固醇会因紫外线照射而发生氧化变化，而所检测的其他脂质（二棕榈酰磷脂酰胆碱、神经酰胺III和硫酸胆固醇）对氧化应激稳定。分析脂质体时观察到了几种脂质加合物。通过MS/MS实验确定了这些加合物的组成。
