Lakeev Iu V, Kosykh V A, Kosenkov E I, Novikov V L, Lebedev A V, Repin V S
Biull Eksp Biol Med. 1992 Nov;114(11):477-80.
Primary cultures of rabbit hepatocytes were used to examine the effect of natural and synthetic antioxidants--polyhydroxynaphthoquinones (PHNQ) and alpha-tocopherol on cholesterol and bile acid synthesis. Histochrome, one of the PHNQ, slightly decreased cholesterol synthesis at concentrations 10-100 microM, whereas alpha-tocopherol stimulated cholesterol synthesis. After administration of histochrome or alpha-tocopherol into culture medium a significant stimulation of bile acid synthesis in dose-dependent manner was observed. The increase of bile acid secretion by histochrome in the presence of physiological concentration of HDL2 was found as well. Since histochrome in contrast to alpha-tocopherol enhanced accumulation of [14C] cholesterol of HDL2 in the hepatocytes, it was concluded that histochrome stimulated bile acid synthesis as a result of increased input of HDL2 cholesterol into hepatocytes. These data suggest that histochrome may exhibit a hypocholesterolemic effect by stimulation of bile acid synthesis and inhibition of cholesterol synthesis.
采用兔肝细胞原代培养物来研究天然和合成抗氧化剂——多羟基萘醌（PHNQ）和α-生育酚对胆固醇及胆汁酸合成的影响。PHNQ之一的组织色素，在浓度为10 - 100微摩尔时，可轻微降低胆固醇合成，而α-生育酚则刺激胆固醇合成。将组织色素或α-生育酚加入培养基后，观察到胆汁酸合成呈剂量依赖性显著增加。还发现，在生理浓度的HDL2存在下，组织色素可增加胆汁酸分泌。由于与α-生育酚相比，组织色素可增强HDL2的[14C]胆固醇在肝细胞中的积累，因此得出结论，组织色素刺激胆汁酸合成是由于HDL2胆固醇进入肝细胞的量增加所致。这些数据表明，组织色素可能通过刺激胆汁酸合成和抑制胆固醇合成而呈现降胆固醇作用。
