Mambetisaeva E T, Kosykh V A, Misharin A Iu, Maliugin A V, Kosenkov E I, Podrez E A, Repin V S
Biokhimiia. 1993 Feb;58(2):261-7.
The effects of 15-ketosterol on cholesterol metabolism in cultured rabbit hepatocytes were characterized by the following parameters: a) cholesterol synthesis, b) apo B and apo E secretion, c) bile acid synthesis. 15-Ketosterol used at therapeutic concentration (0.25 microM) reduced cholesterol synthesis (by 50%). Marked inhibition (by 70%) of apo B and apo E secretion was observed for this agent. Synthesis and secretion of the total [14C]-labeled protein remaining unchanged. 15-Ketosterol did not influence the bile acid synthesis in primary culture of rabbit hepatocytes. These results are suggestive of a new putative mechanism of hyperlipidemic action of 15-ketosterol by a simultaneous decrease of hepatic cholesterol synthesis and secretion of apo B-containing particles.
15-酮固醇对培养的兔肝细胞胆固醇代谢的影响通过以下参数来表征：a）胆固醇合成；b）载脂蛋白B和载脂蛋白E分泌；c）胆汁酸合成。以治疗浓度（0.25微摩尔）使用的15-酮固醇可降低胆固醇合成（降低50%）。观察到该药物对载脂蛋白B和载脂蛋白E的分泌有显著抑制作用（抑制70%）。总的[14C]标记蛋白的合成和分泌保持不变。15-酮固醇不影响兔肝细胞原代培养中的胆汁酸合成。这些结果提示了15-酮固醇导致高脂血症作用的一种新的可能机制，即同时降低肝脏胆固醇合成和含载脂蛋白B颗粒的分泌。
