Glazyrin A L, Kolesnikov S I
Biull Eksp Biol Med. 1992 Nov;114(11):539-41.
Glucose-6-phosphate residues were revealed by fine structural analysis using glucose-6-phosphate dehydrogenase-gold conjugate. In human mature placenta specific staining was detected over the stroma of placental villi. Colloidal gold particles were found over the collagen fibrils and reticular lamina of basal membrane. Syncytiotrophoblast cells, fibroblasts, endothelial cells of fetal capillaries avoided labelling. Nucleated blood cells and thrombocytes inside the lumen of fetal capillaries demonstrated intense staining. The present investigation demonstrates histochemically the process of extracellular glycosylation. Glycated collagen fibrils formed channels inside the stroma of placental villi.
使用葡萄糖-6-磷酸脱氢酶-金偶联物通过精细结构分析揭示了葡萄糖-6-磷酸残基。在人成熟胎盘中，在胎盘绒毛的基质上检测到特异性染色。在基底膜的胶原纤维和网状层上发现了胶体金颗粒。合体滋养层细胞、成纤维细胞、胎儿毛细血管内皮细胞未被标记。胎儿毛细血管腔内的有核血细胞和血小板显示出强烈的染色。本研究通过组织化学方法证明了细胞外糖基化过程。糖化的胶原纤维在胎盘绒毛基质内形成通道。
