Iida K, Kobayashi M, Kobayashi K, Saga S, Hoshino T, Matsuyama M
Department of Pathology, Nagoya University School of Medicine, Japan.
J Electron Microsc (Tokyo). 1994 Dec;43(6):367-72.
The distribution of type VI collagen in the chorionic villi of normal human term placenta was studied. Since 100 nm periodic fibrils (type VI collagen periodic fibrils) are known to form in the tendon, cornea and cultured fibroblasts by incubation with acidic ATP solution, we incubated the placental tissue with 20 mM ATP, fixed it with Karnovsky's fixative and examined under an electron microscope. Type VI collagen periodic fibrils widely occurred in the interstitial connective tissue, especially close to the basal lamina. By cryo-immunoelectron microscopy, labeling for collagen type VI was found in the area between the trophoblastic basal lamina and the endothelial basal lamina. Furthermore, we extracted type VI collagen from the human placenta and brought both extract and residues into ATP treatment. Periodic structure formation was rare in extracted preparation compared with residues. The results served to clarify the mechanism of fibril formation in the tissue.
研究了正常人足月胎盘绒毛膜中VI型胶原的分布。由于已知通过用酸性ATP溶液孵育，在肌腱、角膜和培养的成纤维细胞中会形成100nm周期性纤维（VI型胶原周期性纤维），我们用20mM ATP孵育胎盘组织，用卡诺夫斯基固定液固定，并在电子显微镜下检查。VI型胶原周期性纤维广泛存在于间质结缔组织中，尤其是靠近基膜处。通过冷冻免疫电子显微镜观察，在滋养层基膜和内皮基膜之间的区域发现了VI型胶原的标记。此外，我们从人胎盘中提取了VI型胶原，并对提取物和残渣都进行了ATP处理。与残渣相比，提取物制剂中很少形成周期性结构。这些结果有助于阐明组织中纤维形成的机制。
