Kasili P M, Cullum B M, Griffin G D, Vo-Dinh T
Advanced Biomedical Science and Technology Group, Life Sciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.
J Nanosci Nanotechnol. 2002 Dec;2(6):653-8. doi: 10.1166/153348802321105941.
This work describes the fabrication and the application of an antibody-based fiber-optic nanosensor for in situ measurements of the carcinogen benzo[a]pyrene (BaP) in a single cell. This antibody-based spectroscopic nanosensor is miniaturized enabling the detection of fluorescent analytes in single cells. In addition to measuring fluorescent analytes in single cells, the nanosensor has the potential to be applied for both diagnostic and proteomics purposes. In this work, the human breast carcinoma cell line, MCF-7, was used as the model system to perform BaP measurements in single cells. A standard concentration curve for BaP was established and used to perform quantitative analyses of BaP in individual cells. From these analyses, it was estimated that the concentration of BaP in the individual cells investigated was approximately 3.61 x 10(-10) M. The results obtained demonstrate the application of antibody-based nanosensors for performing in situ measurements inside a single cell.
这项工作描述了一种基于抗体的光纤纳米传感器的制造及其应用，该传感器用于原位测量单个细胞中的致癌物苯并[a]芘（BaP）。这种基于抗体的光谱纳米传感器实现了小型化，能够检测单个细胞中的荧光分析物。除了测量单个细胞中的荧光分析物外，该纳米传感器还具有用于诊断和蛋白质组学目的的潜力。在这项工作中，人类乳腺癌细胞系MCF-7被用作模型系统，以在单个细胞中进行BaP测量。建立了BaP的标准浓度曲线，并用于对单个细胞中的BaP进行定量分析。通过这些分析，估计所研究的单个细胞中BaP的浓度约为3.61×10（-10）M。所获得的结果证明了基于抗体的纳米传感器在单个细胞内进行原位测量的应用。
