Vo-Dinh Tuan, Kasili Paul
Oak Ridge National Laboratory, Advanced Biomedical Science and Technology Group, Oak Ridge, TN 37831-6101, USA.
Anal Bioanal Chem. 2005 Jun;382(4):918-25. doi: 10.1007/s00216-005-3256-7. Epub 2005 Jun 1.
This article is an overview of the fabrication, operating principles, and applications of fiber-optic nanobiosensors with the capability of in-vivo analysis at the single-cell level. Recently, the cross-disciplinary integration of nanotechnology, biology, and photonics has been revolutionizing important areas in molecular biology, especially diagnostics and therapy at the molecular and cellular level. Fiber-optic nanobiosensors are a unique class of biosensor that enable analytical measurements in individual living cells and the probing of individual chemical species in specific locations within a cell. This article provides a review of the research performed in our laboratory and discusses the usefulness and potential of this nanotechnology-based biosensor system in biological research and its applications to biomonitoring of individual cells.
本文概述了具有单细胞水平体内分析能力的光纤纳米生物传感器的制造、工作原理和应用。近年来，纳米技术、生物学和光子学的跨学科整合一直在彻底改变分子生物学的重要领域，尤其是分子和细胞水平的诊断与治疗。光纤纳米生物传感器是一类独特的生物传感器，能够在单个活细胞中进行分析测量，并探测细胞内特定位置的单个化学物质。本文回顾了我们实验室开展的研究，并讨论了这种基于纳米技术的生物传感器系统在生物学研究中的实用性和潜力及其在单个细胞生物监测中的应用。
