Kullisaar Tiiu, Songisepp Epp, Mikelsaar Marika, Zilmer Kersti, Vihalemm Tiiu, Zilmer Mihkel
Department of Biochemistry, Medical Faculty, University of Tartu, Tartu, Estonia.
Br J Nutr. 2003 Aug;90(2):449-56. doi: 10.1079/bjn2003896.
The increasing interest in a healthy diet is stimulating innovative development of novel scientific products in the food industry. The viable lactic acid bacteria in fermented milk products, such as yoghurt, have been associated with increased lactose tolerance, a well-balanced intestinal microflora, antimicrobial activity, stimulation of the immune system and antitumoural, anticholesterolaemic and antioxidative properties in human subjects. Recently, we have studied a human Lactobacillus spp. strain that possesses antioxidative activity. The aim of the present pilot study was to develop goats' milk fermented with the human antioxidative lactobacilli strain, Lactobacillus fermentum ME-3, and to test the effect of the fermented probiotic goats' milk on oxidative stress markers (including markers for atherosclerosis) in human blood and urine and on the gut microflora. Twenty-one healthy subjects were assigned to two treatment groups: goats' milk group and fermented goats' milk group (150 g/d) for a period of 21 d. Consumption of fermented goats' milk improved anti-atherogenicity in healthy subjects: it prolonged resistance of the lipoprotein fraction to oxidation, lowered levels of peroxidized lipoproteins, oxidized LDL, 8-isoprostanes and glutathione redox ratio, and enhanced total antioxidative activity. The consumption of fermented goats' milk also altered both the prevalence and proportion of lactic acid bacteria species in the gut microflora of the subjects. We conclude that the goats' milk fermented with our special antioxidative lactobacilli strain Lactobacillus fermentum ME-3 exhibits anti-atherogenic effects.
人们对健康饮食的兴趣日益浓厚，这刺激了食品行业新型科学产品的创新发展。发酵乳制品（如酸奶）中的活性乳酸菌与提高乳糖耐受性、肠道微生物群平衡、抗菌活性、免疫系统刺激以及人体的抗肿瘤、抗胆固醇和抗氧化特性有关。最近，我们研究了一种具有抗氧化活性的人类乳酸杆菌菌株。本初步研究的目的是开发用人抗氧化乳酸菌菌株发酵乳杆菌ME-3发酵的山羊奶，并测试发酵益生菌山羊奶对人体血液和尿液中的氧化应激标志物（包括动脉粥样硬化标志物）以及肠道微生物群的影响。21名健康受试者被分为两个治疗组：山羊奶组和发酵山羊奶组（每天150克），为期21天。食用发酵山羊奶可改善健康受试者的抗动脉粥样硬化能力：它延长了脂蛋白部分对氧化的抵抗力，降低了过氧化脂蛋白、氧化低密度脂蛋白、8-异前列腺素和谷胱甘肽氧化还原比的水平，并增强了总抗氧化活性。食用发酵山羊奶还改变了受试者肠道微生物群中乳酸菌种类的患病率和比例。我们得出结论，用我们特殊的抗氧化乳酸菌菌株发酵乳杆菌ME-3发酵的山羊奶具有抗动脉粥样硬化作用。
