Vuorela Piia, Lintula Susanna, Stenman Ulf-Håkan, Halmesmäki Erja
Department of Obstetrics and Gynecology, Helsinki University Central Hospital, Biomedicum, Helsinki, Finland.
Hypertens Pregnancy. 2003;22(2):193-201. doi: 10.1081/PRG-120021065.
Preeclampsia is associated with platelet and endothelial dysfunction. Vascular endothelial growth factor (VEGF) is found in peripheral blood leukocytes and released from platelets on activation. We analyzed the content of (VEGF) in peripheral blood cells of preeclamptic women.
The VEGF content of platelets, mononuclear white blood cells, and granulocytes of peripheral blood were analyzed from 12 women with preeclampsia, 19 healthy pregnant women, and 20 nonpregnant women. Protein released from lysed cells was analyzed by enzyme-linked immunoassay (ELISA).
Platelet VEGF content of preeclamptic women (0.081 ng/109 cells, 0.016 to 2.7 ng/109 cells; median, range) was similar to that of healthy pregnant women (0.31 ng/109 cells, 0.013 to 0.92 ng/109 cells) and that of nonpregnant (0.073 ng/109 cells, 0.012 to 0.76 ng/109 cells) women. Likewise, the VEGF content of granulocytes was similar in preeclamptic (18.5 ng/109 cells, 1.2 to 193 ng/109 cells), healthy pregnant (25.3 ng/109 cells, 0.8 to 441 ng/109 cells), and nonpregnant (29 ng/109 cells, 0.25 to 200 ng/109 cells) women. In mononuclear cells, the VEGF content of healthy pregnant women was higher (4.4 ng/109 cells, 0.13 to 13.7 ng/109 cells) than in nonpregnant women (1.7 ng/109 cells, 0.15 to 11.4 ng/109 cells, P < 0.05). Also, the mononuclear cell VEGF content of preeclamptic women (8.2 ng/109 cells, 0.04 to 23 ng/109 cells) tended to be higher than in nonpregnant women (P approximately 0.07).
Uncomplicated pregnancy is associated with an elevated VEGF content of mononuclear cells. Preeclampsia does not seem to affect the VEGF content of maternal peripheral blood mononuclear cells, granulocytes, or platelets.
子痫前期与血小板及内皮功能障碍有关。血管内皮生长因子（VEGF）存在于外周血白细胞中，并在血小板激活时释放。我们分析了子痫前期女性外周血细胞中VEGF的含量。
分析了12例子痫前期女性、19例健康孕妇和20例非孕妇外周血中血小板、单核白细胞和粒细胞的VEGF含量。通过酶联免疫吸附测定（ELISA）分析裂解细胞释放的蛋白质。
子痫前期女性血小板VEGF含量（0.081 ng/10⁹细胞，0.016至2.7 ng/10⁹细胞；中位数，范围）与健康孕妇（0.31 ng/10⁹细胞，0.013至0.92 ng/10⁹细胞）及非孕妇（0.073 ng/10⁹细胞，0.012至0.76 ng/10⁹细胞）相似。同样，子痫前期女性（18.5 ng/10⁹细胞，1.2至193 ng/10⁹细胞）、健康孕妇（25.3 ng/10⁹细胞，0.8至441 ng/10⁹细胞）和非孕妇（29 ng/10⁹细胞，0.25至200 ng/10⁹细胞）粒细胞中的VEGF含量相似。在单核细胞中，健康孕妇的VEGF含量（4.4 ng/10⁹细胞，0.13至13.7 ng/10⁹细胞）高于非孕妇（1.7 ng/10⁹细胞，0.15至11.4 ng/10⁹细胞，P<0.05）。此外，子痫前期女性单核细胞VEGF含量（8.2 ng/10⁹细胞，0.04至23 ng/10⁹细胞）也倾向于高于非孕妇（P约为0.07）。
正常妊娠与单核细胞VEGF含量升高有关。子痫前期似乎不影响母体外周血单核细胞、粒细胞或血小板的VEGF含量。
