Oka Kuniyuki, Suzuki Yoshiyuki, Iida Hiroko, Nakano Takashi
Department of Pathology, Mito Saiseikai General Hospital, Mito, Ibaraki, Japan.
Int J Radiat Oncol Biol Phys. 2003 Sep 1;57(1):217-21. doi: 10.1016/s0360-3016(03)00436-x.
Platelet-derived endothelial cell growth factor (PD-ECGF), which has angiogenic activity, is identical to thymidine phosphorylase. Tumor vascularization is considered to be an important prognostic factor. Nitric oxide synthases (NOSs) are a kind of enzyme that generates nitric oxide. Nitric oxide has not only a self defense against neoplastic cells but also tumor growth stimulation by promoting new blood vessel formation. Our purpose was to investigate the correlation between the expression of PD-ECGF or inducible NOS (iNOS) in cancer cells and prognosis.
Formaldehyde-fixed and paraffin-embedded biopsy specimens excised from 71 cervical squamous cell carcinoma patients who were treated with radiotherapy alone were investigated using an immunohistochemical method.
Cancer cells that were positive for PD-ECGF showed intranuclear and cytoplasmic staining patterns. Of the 71 patients, 40 (56%) were positive for PD-ECGF and 31 (44%) were negative. The 5-year survival of the PD-ECGF-positive patients was significantly better than that of the PD-ECGF-negative patients (p = 0.026). Cancer cells that were positive for iNOS showed a cytoplasmic staining pattern. Twenty-seven patients (38%) were positive for iNOS and 44 (62%) were negative. No significant prognostic correlation was observed between iNOS-positive and iNOS-negative patients.
PD-ECGF positivity in cancer cells is a predictive factor for a good prognosis in cervical squamous cell carcinoma treated with radiotherapy alone.
具有血管生成活性的血小板衍生内皮细胞生长因子（PD-ECGF）与胸苷磷酸化酶相同。肿瘤血管生成被认为是一个重要的预后因素。一氧化氮合酶（NOSs）是一种产生一氧化氮的酶。一氧化氮不仅具有对肿瘤细胞的自身防御作用，还通过促进新血管形成刺激肿瘤生长。我们的目的是研究癌细胞中PD-ECGF或诱导型NOS（iNOS）的表达与预后之间的相关性。
使用免疫组织化学方法对71例仅接受放射治疗的宫颈鳞状细胞癌患者切除的甲醛固定、石蜡包埋活检标本进行研究。
PD-ECGF阳性的癌细胞呈现核内和胞质染色模式。71例患者中，40例（56%）PD-ECGF阳性，31例（44%）阴性。PD-ECGF阳性患者的5年生存率显著高于PD-ECGF阴性患者（p = 0.026）。iNOS阳性的癌细胞呈现胞质染色模式。27例患者（38%）iNOS阳性，44例（62%）阴性。iNOS阳性和阴性患者之间未观察到显著的预后相关性。
癌细胞中PD-ECGF阳性是仅接受放射治疗的宫颈鳞状细胞癌预后良好的预测因素。
