Cesari Michele, Luchetti Andrea, Passamonti Marco, Scali Valerio, Mantovani Barbara
Dipartimento di Biologia Evoluzionistica Sperimentale, Università di Bologna, Via Selmi 3, 40126, Bologna, Italy.
Gene. 2003 Jul 17;312:289-95. doi: 10.1016/s0378-1119(03)00625-5.
Polymerase chain reaction amplifications of genomic DNA in 17 individuals of bisexual and parthenogenetic populations of three subspecies of Bacillus rossius (Insecta Phasmatodea) revealed that the species still harbours the whole variability of the ancestral Bag320 satellite family, since monomers of all non-hybrid Bacillus taxa plus private sequences occur in it. Bag320 monomers had not been rescued as a major satellite component in B. rossius, but possibly represent the remnant of a set of diverging sequences present in the Bacillus ancestor. Following the library hypothesis, these monomer variants have been differently amplified along the evolutionary pathways leading to present taxa in agreement with the mitochondrial phylogeny of the genus. The putative converted tracts observed are explained as the results of past gene conversion events.
对罗斯氏竹节虫（昆虫纲竹节虫目）三个亚种的17个两性生殖和孤雌生殖种群的基因组DNA进行聚合酶链反应扩增，结果显示该物种仍然保留着祖先Bag320卫星家族的全部变异性，因为所有非杂交的竹节虫分类单元的单体以及特有序列都存在于其中。Bag320单体在罗斯氏竹节虫中并未作为主要卫星成分被拯救出来，但可能代表了竹节虫祖先中一组发散序列的残余。根据文库假说，这些单体变体在导致当前分类单元的进化途径中沿着与该属线粒体系统发育一致的方向被不同程度地扩增。观察到的推定转换区域被解释为过去基因转换事件的结果。
