Gianaroli Luca, Magli M Cristina, Ferraretti Anna P, Fortini Daniela, Grieco Natascia
Società Italiana Studi Medicina della Riproduzione, Reproductive Medicine Unit, Bologna, Italy.
Fertil Steril. 2003 Aug;80(2):341-9. doi: 10.1016/s0015-0282(03)00596-x.
To verify whether a correlation exists between pronuclear zygote morphology and the chromosomal condition of preimplantation embryos.
Prospective analysis of pronuclear zygote morphology and preimplantation genetic diagnosis (PGD) for aneuploidy of the resulting embryos.
Reproductive medicine unit, day surgery clinic.
PATIENT(S): Seventy-seven patients undergoing 107 PGD cycles because of advanced maternal age (77 cycles) or previous IVF failures (30 cycles).
INTERVENTION(S): Evaluation of pronuclear zygote morphology and chromosomal condition of the resulting embryos.
MAIN OUTCOME MEASURE(S): Rate of embryo development, proportion of euploid embryos, and distribution of chromosomal abnormalities. The position of pronuclei within the ooplasm, the size and distribution of nucleoli, and the orientation of polar bodies with respect to pronuclei were highly predictive for the presence of complex chromosomal abnormalities in the developing embryos; zygotes with juxtaposed pronuclei, large-size nucleoli, and polar bodies with small angles subtended by pronuclei and polar bodies were the configurations associated with the highest rates of euploidy.
CONCLUSION(S): The combination of the patterns related to pronuclear zygote morphology indicated four configurations where the proportion of chromosomally normal embryos was significantly higher compared with the other configurations, suggesting the validity of this scoring system for the selection of embryos generated by PGD patients.
验证原核受精卵形态与植入前胚胎染色体状况之间是否存在相关性。
对原核受精卵形态及由此产生的胚胎进行非整倍体植入前基因诊断（PGD）的前瞻性分析。
日间手术诊所生殖医学科。
77例患者因高龄产妇（77个周期）或既往体外受精失败（30个周期）接受了107个PGD周期治疗。
评估原核受精卵形态及由此产生的胚胎的染色体状况。
胚胎发育率、整倍体胚胎比例及染色体异常分布。原核在卵质内的位置、核仁的大小和分布以及极体相对于原核的方向对发育中胚胎复杂染色体异常的存在具有高度预测性；原核并列、核仁大以及极体与原核所夹角度小的受精卵是整倍体率最高的构型。
与原核受精卵形态相关的模式组合表明有四种构型产生的染色体正常胚胎比例显著高于其他构型，提示该评分系统对PGD患者所产生胚胎的选择具有有效性。
