Abir Ronit, Orvieto Raoul, Raanani Hila, Feldberg Dov, Nitke Shmuel, Fisch Benjamin
Department of Obstetrics and Gynecology, Rabin Medical Center, Beilinson Campus, Petah Tikva, Israel.
Fertil Steril. 2003 Aug;80(2):421-8. doi: 10.1016/s0015-0282(03)00658-7.
To compare the development and survival of human fetal follicles frozen-thawed with dimethylsulfoxide (DMSO) and propandiol (PROH) in immunodeficient mice, to study the effects of host treatment with FSH, and to compare kidney and subcutaneous transplantation.
Controlled histologic study.
Major tertiary care and referral academic center.Twenty-one women undergoing second-trimester pregnancy termination. Microscopic morphometric analysis and immunocytochemistry for proliferating-cell nuclear antigen in human fetal ovaries grafted into immunodeficient mice.
Renal grafts that were frozen-thawed with DMSO rather than PROH survived better in the hosts (79.6% compared with 58.8%), but significantly more follicles were identified in grafts frozen-thawed with PROH (P<.001). Follicular development was observed only in FSH-treated hosts, and follicular survival and development was better in the kidney than the subcutaneous site.
CONCLUSION(S): This is the first report showing development of human fetal follicles in immunodeficient mice. Freezing-thawing with PROH seems to support development and survival better than with DMSO. The kidney is a better transplantation site than the subcutaneous site, probably because of its superior vascularization. Administration of FSH to the host is essential for follicular development. Follicular development and growth was better in ovarian grafts from older fetuses, as they contained more formed follicles.
比较用二甲基亚砜（DMSO）和丙二醇（PROH）冻融的人胎儿卵泡在免疫缺陷小鼠体内的发育和存活情况，研究用促卵泡激素（FSH）处理宿主的效果，并比较肾脏移植和皮下移植。
对照组织学研究。
大型三级医疗和转诊学术中心。21名接受孕中期引产的妇女。对移植到免疫缺陷小鼠体内的人胎儿卵巢进行显微镜形态计量分析和增殖细胞核抗原免疫细胞化学分析。
用DMSO而非PROH冻融的肾脏移植物在宿主中的存活率更高（79.6%对58.8%），但用PROH冻融的移植物中发现的卵泡明显更多（P<0.001）。仅在经FSH处理的宿主中观察到卵泡发育，且肾脏部位的卵泡存活和发育情况优于皮下部位。
这是首次报道人胎儿卵泡在免疫缺陷小鼠体内发育的研究。与DMSO相比，用PROH冻融似乎更有利于卵泡的发育和存活。肾脏是比皮下更好的移植部位，可能是因为其血管化程度更高。对宿主给予FSH对卵泡发育至关重要。来自较大胎儿的卵巢移植物中卵泡发育和生长情况更好，因为它们含有更多已形成的卵泡。
