Choi Won-Jun, Seok Jin-Sook, Choi In-Young, Park Ji-Kwon, Shin Jeong-Kyu, Lee Soon-Ae, Paik Won-Young, Lee Jong-Hak
Department of Obstetrics and Gynecology, School of Medicine, Gyeongsang National University, Jinju, Korea. ; Institute of Health Sciences, Gyeongsang National University, Jinju, Korea.
Department of Obstetrics and Gynecology, School of Medicine, Gyeongsang National University, Jinju, Korea.
Obstet Gynecol Sci. 2015 Sep;58(5):391-6. doi: 10.5468/ogs.2015.58.5.391. Epub 2015 Sep 22.
Revascularization is critical for successful ovarian tissue transplantation. Vascular endothelial growth factor (VEGF) and angiopoietin-2 (angpt-2) are the principal mediators of neovascularization. This study was designed to assess VEGF and angpt-2 levels in cryopreserved ovarian tissue after heterotopic autotransplantation.
Ovarian tissues harvested from ICR mice at 5 to 6 weeks of age were stratified as follows: no cryopreservation (controls, group I); vitrification in VFS-40 (vitrification, group II); and gradual freezing in dimethyl sulfoxide (slow-freezing, group III). Frozen specimens were thawed at room temperature, assaying VEGF and angpt-2 levels 1 week after cryopreservation and 2 weeks after autotransplantation.
VEGF and angpt-2 protein levels were significantly lower in cryopreserved ovaries of groups II and III than in controls (group I, P<0.05), whereas groups II and III did not differ significantly in this regard. After autotransplantation of cryopreserved ovarian tissue, VEGF and angpt-2 protein levels did not differ significantly by technique but tended to be lower than corresponding levels in controls.
Expression of angiogenic factors in ovarian tissue is thought to vary by method of cryopreservation. Our findings indicate that levels of angiogenic factors expressed in cryopreserved ovarian tissue after autotransplantation do not differ appreciably from control levels, regardless of cryopreservation technique.
血管重建对于成功进行卵巢组织移植至关重要。血管内皮生长因子（VEGF）和血管生成素-2（angpt-2）是新生血管形成的主要介质。本研究旨在评估异位自体移植后冷冻保存的卵巢组织中VEGF和angpt-2的水平。
从5至6周龄的ICR小鼠采集的卵巢组织分层如下：未冷冻保存（对照组，I组）；在VFS-40中玻璃化（玻璃化组，II组）；以及在二甲亚砜中逐步冷冻（慢速冷冻组，III组）。冷冻标本在室温下解冻，在冷冻保存后1周和自体移植后2周测定VEGF和angpt-2水平。
II组和III组冷冻保存的卵巢中VEGF和angpt-2蛋白水平显著低于对照组（I组，P<0.05），而II组和III组在这方面无显著差异。冷冻保存的卵巢组织自体移植后，VEGF和angpt-2蛋白水平在技术上无显著差异，但往往低于对照组的相应水平。
卵巢组织中血管生成因子的表达被认为因冷冻保存方法而异。我们的研究结果表明，无论冷冻保存技术如何，自体移植后冷冻保存的卵巢组织中血管生成因子的表达水平与对照水平无明显差异。
