半知菌黄曲霉分泌的胶原水解酶的特性

Characteristics of collagenolytic enzymes secreted by deuteromycete fungi Aspergillus flavus.

作者信息

Sukhosyrova E A, Nikitina Z K, Yakovleva M B, Veshchikova E V, Bykov V A

机构信息

Research and Training and Methodical Center for Biomedical Technologies VILAR, Moscow.

出版信息

Bull Exp Biol Med. 2003 May;135(5):447-51. doi: 10.1023/a:1024911308278.

DOI:10.1023/a:1024911308278

PMID:12910283

Abstract

Collagen induced the synthesis of collagenolytic enzymes in the culture of Aspergillus flavus. Enzyme activity of the culture increased during storage and passage on a medium with this inductor. We developed a method for isolation and purification of collagenolytic enzymes and obtained two electrophoretically homogenous enzyme preparations belonging to neutral thermolabile collagenolytic metalloproteinases.

摘要

胶原蛋白在黄曲霉培养物中诱导了胶原分解酶的合成。培养物的酶活性在含有这种诱导剂的培养基上储存和传代过程中有所增加。我们开发了一种分离和纯化胶原分解酶的方法，并获得了两种属于中性热不稳定胶原分解金属蛋白酶的电泳纯酶制剂。