Van Winkle S C, Johnson S, Pullman G S
Institute of Paper Science and Technology, 500 10th Street NW, Atlanta, GA 30318, USA.
Plant Cell Rep. 2003 Aug;21(12):1175-82. doi: 10.1007/s00299-003-0637-2. Epub 2003 May 13.
The elemental composition of plant tissue culture media was studied in response to (1) different levels of Gelrite and activated carbon (AC) in semisolid media and (2) different levels and types of AC in liquid media. Doubling the level of Gelrite from 2 g/l to 4 g/l reduced available magnesium (20%), calcium (16%), zinc (17%) and manganese (24%) and increased potassium (6%). AC adsorbed copper (90-95%) and zinc (35-51%) from both liquid and semisolid media. Two significantly different ACs gave minor differences in adsorption. No adsorption was indicated for inorganic anions. Nonacid-washed AC released significant levels of magnesium (44% increase), calcium (16% increase) and silica (a 75% increase to 1.8 mg/l). The elemental composition of media may need to be adjusted when increasing the Gelrite level or adding AC.
研究了植物组织培养基的元素组成，以应对以下两种情况：（1）半固体培养基中不同水平的结冷胶和活性炭（AC）；（2）液体培养基中不同水平和类型的AC。将结冷胶水平从2 g/l加倍至4 g/l，会使可利用的镁（降低20%）、钙（降低16%）、锌（降低17%）和锰（降低24%）减少，并使钾增加（增加6%）。AC从液体和半固体培养基中吸附铜（90 - 95%）和锌（35 - 51%）。两种差异显著的AC在吸附方面存在细微差异。未发现对无机阴离子有吸附作用。未酸洗的AC释放出大量的镁（增加44%）、钙（增加16%）和硅（增加至1.8 mg/l，增幅75%）。当提高结冷胶水平或添加AC时，可能需要调整培养基的元素组成。
