Pullman G S, Namjoshi K, Zhang Y
Institute of Paper Science and Technology, 500 10th Street, Atlanta, GA 30318, USA.
Plant Cell Rep. 2003 Sep;22(2):85-95. doi: 10.1007/s00299-003-0673-y. Epub 2003 Jul 19.
Loblolly pine ( Pinus taeda L.) culture initiation was improved by the addition of abscisic acid (ABA) (3.7 micro M), silver nitrate (20 micro M), and guanosine 3',5'-cyclic monophosphate, 8-bromo-, sodium salt (10 micro M) to the medium and by raising cytokinin levels in the presence of 50 mg/l activated carbon (AC). Basal medium contained modified 1/2-P6 salts, 50 mg/l AC, Cu and Zn added to compensate for adsorption by AC, 1.5% maltose, 2% myo-inositol, 500 mg/l casamino acids, 450 mg/l glutamine, 2 mg/l alpha-naphthaleneacetic acid (NAA), 0.55 mg/l 6-benzylaminopurine (BA), 0.53 mg/l kinetin, and 2 g/l Gelrite. Across 32 open-pollinated families initiation ranged from 0 to 53.4%, with an average of 17.9%. Further optimization of cytokinins to 0.63 mg/l BA and 0.61 mg/l kinetin along with the removal of ABA maintained initiation at 18.2% across 19 families. Survival of 2001 new initiations was tracked for 4-6 months. Survival averaged 28.8%. A test of 68 new initiations tracked closely for 4 months demonstrated that at least 80% of the cultures lost did not grow after transfer to the multiplication media, suggesting that many new initiations abort during the initiation process.
通过在培养基中添加脱落酸（ABA）（3.7微摩）、硝酸银（20微摩）和8-溴-3',5'-环磷酸鸟苷钠盐（10微摩），并在50毫克/升活性炭（AC）存在的情况下提高细胞分裂素水平，火炬松（Pinus taeda L.）的培养起始率得到了改善。基础培养基含有改良的1/2-P6盐、50毫克/升AC、添加的铜和锌以补偿AC的吸附作用、1.5%麦芽糖、2%肌醇、500毫克/升酪蛋白氨基酸、450毫克/升谷氨酰胺、2毫克/升α-萘乙酸（NAA）、0.55毫克/升6-苄基腺嘌呤（BA）、0.53毫克/升激动素和2克/升吉丽替。在32个开放授粉家系中，起始率范围为0至53.4%，平均为17.9%。将细胞分裂素进一步优化至0.63毫克/升BA和0.61毫克/升激动素，并去除ABA，19个家系的起始率维持在18.2%。对2001个新起始培养物进行了4至6个月的存活跟踪。平均存活率为28.8%。对68个新起始培养物进行了4个月的密切跟踪测试，结果表明，转移至增殖培养基后，至少80%死亡的培养物没有生长，这表明许多新起始培养物在起始过程中就夭折了。
