Debray H, Wieruszeski J M, Strecker G, Franz H
Laboratoire de Chimie Biologique, Unité Mixte de Recherche du CNRS No. 111, Université des Sciences et Techniques de Lille Flandres-Artois, Villeneuve d'Ascq, France.
Carbohydr Res. 1992 Dec 15;236:135-43. doi: 10.1016/0008-6215(92)85012-o.
Two glycopeptide fractions prepared from mistletoe (Viscum album) lectin I by Pronase digestion were fractioned by affinity chromatography on a concanavalin A-Sepharose column. With 400-MHz 1H NMR spectroscopy, in conjunction with sugar analysis, the following oligosaccharide structures could be determined: two oligomannose-type glycans in the ratio 4:1, one containing six mannose and the other containing five mannose units, both with two 2-acetamido-2-deoxyglucose units. In addition, a mannotriosyl-->N,N'-diacetylchitobiose glycan containing a xylosyl group and an alpha-fucosyl group (1-->3)-linked to the 2-acetamido-2-deoxyglycosyl-1 residue, a common core element of many plant glycoproteins, was also observed.
通过链霉蛋白酶消化从欧洲槲寄生（Viscum album）凝集素I制备的两个糖肽级分，在伴刀豆球蛋白A-琼脂糖柱上进行亲和色谱分离。利用400兆赫的1H核磁共振光谱，并结合糖分析，可确定以下寡糖结构：两种低聚甘露糖型聚糖，比例为4:1，一种含有六个甘露糖，另一种含有五个甘露糖单元，两者均含有两个2-乙酰氨基-2-脱氧葡萄糖单元。此外，还观察到一种含有木糖基和（1→3）连接到2-乙酰氨基-2-脱氧糖基-1残基上的α-岩藻糖基的甘露三糖→N，N'-二乙酰壳二糖聚糖，这是许多植物糖蛋白的常见核心元件。
