Sturm A, Bergwerff A A, Vliegenthart J F
Friedrich Miescher-Institute, Basel, Switzerland.
Eur J Biochem. 1992 Feb 15;204(1):313-6. doi: 10.1111/j.1432-1033.1992.tb16639.x.
Phytohemagglutinin, the lectin of the common bean Phaseolus vulgaris, is a N-linked glycoprotein with one high-mannose-type and one xylose-containing oligosaccharide side chain per polypeptide. The high-mannose-type glycan is attached to Asn12 and the complex-type glycan to Asn60 [Sturm, A. & Chrispeels, M. J. (1986) Plant Physiol. 81, 320-322]. The structures of the oligosaccharides were elucidated from two glycopeptides obtained from the lectin by Pronase digestion, affinity chromatography on concanavalin-A--Sepharose and gel-filtration chromatography on a column of BioGel P-4. The N-linked glycan structures were investigated by 500-MHz 1H-NMR spectroscopy and were established to be: [formula; see text]
植物血凝素是普通菜豆（Phaseolus vulgaris）的凝集素，是一种N-连接糖蛋白，每个多肽含有一个高甘露糖型和一个含木糖的寡糖侧链。高甘露糖型聚糖连接到Asn12，复合型聚糖连接到Asn60 [Sturm, A. & Chrispeels, M. J. (1986) Plant Physiol. 81, 320 - 322]。通过链霉蛋白酶消化从凝集素获得的两个糖肽、伴刀豆球蛋白A - 琼脂糖亲和色谱和BioGel P - 4柱凝胶过滤色谱法阐明了寡糖的结构。通过500兆赫的1H - NMR光谱研究了N - 连接聚糖结构，确定为：[分子式；见正文]
