Yilmaz Okkeş, Celik Sait
Department of Biology, Faculty of Science, Firat University, Elazig, Turkey.
Cell Biochem Funct. 2003 Sep;21(3):269-74. doi: 10.1002/cbf.1022.
This research was aimed at evaluating the antioxidant effects of combinations of alpha lipoic acid (LA), vitamin C (VC), N-acetyl cysteine (NAC) and alpha-tocopherol (TOC) on lipid level and fatty acid composition of C. tropicalis (ATCC 13803) against hydrogen peroxide toxicity. According to the experimental results, the cell density of C. tropicalis increased significantly in NAC+LA+H2O2, NAC+TOC+ H2O2 and NAC+VC+H2O2 groups (p<0.001) at the end of 48 and 72 h incubation times. The total lipid level in H2O2 and H2O2 + antioxidant-supplemented groups was lower than that of the control group. In the fatty acid composition of C. tropicalis, the palmitic acid level was raised in the NAC group (p<0.05), whereas its level was reduced in the other supplemented groups. While the oleic acid level increased in NAC+TOC+H2O2 and NAC+VC+H2O2 (p<0.001) groups, its level slightly decreased in the H2O2 group. The linolenic acid level was low in all the supplemented groups, but linoleic acid and total mono-unsaturated fatty acid (MUFA) levels were high in these groups compared with the control group. Total polyunsaturated fatty acid level (PUFA) decreased in NAC and H2O2 groups (p<0.01), but its level increased in NAC+LA+H2O2 and NAC+TOC+H2O2 groups (respectively, p<0.01, p<0.001). Total saturated fatty acid level decreased significantly in NAC+TOC+H2O2, NAC+H2O2 and NAC+VC+H2O2 (p<0.001) groups (p<0.01), whereas total unsaturated fatty acid level increased in NAC, NAC+H2O2, NAC+LA+H2O2, NAC+TOC+H2O2 and NAC+VC+H2O2 groups. In conclusion, our data showed that the levels of total unsaturated fatty acid, MUFA and PUFA were raised with the combinations of NAC and TOC, LA and VC in C. tropicalis cells subjected to hydrogen peroxide toxicity.
本研究旨在评估α-硫辛酸（LA）、维生素C（VC）、N-乙酰半胱氨酸（NAC）和α-生育酚（TOC）组合对热带假丝酵母（ATCC 13803）脂质水平和脂肪酸组成的抗氧化作用，以对抗过氧化氢毒性。根据实验结果，在48小时和72小时孵育结束时，NAC+LA+H2O2、NAC+TOC+H2O2和NAC+VC+H2O2组中热带假丝酵母的细胞密度显著增加（p<0.001）。H2O2组和添加抗氧化剂的H2O2组中的总脂质水平低于对照组。在热带假丝酵母的脂肪酸组成中，NAC组中棕榈酸水平升高（p<0.05），而在其他添加组中其水平降低。虽然NAC+TOC+H2O2和NAC+VC+H2O2组中油酸水平升高（p<0.001），但在H2O2组中其水平略有下降。所有添加组中亚麻酸水平较低，但与对照组相比，这些组中亚油酸和总单不饱和脂肪酸（MUFA）水平较高。NAC组和H2O2组中总多不饱和脂肪酸水平（PUFA）降低（p<0.01），但在NAC+LA+H2O2和NAC+TOC+H2O2组中其水平升高（分别为p<0.01，p<0.001）。NAC+TOC+H2O2、NAC+H2O2和NAC+VC+H2O2组中总饱和脂肪酸水平显著降低（p<0.001）（p<0.01），而NAC、NAC+H2O2、NAC+LA+H2O2、NAC+TOC+H2O2和NAC+VC+H2O2组中总不饱和脂肪酸水平升高。总之，我们的数据表明，在受到过氧化氢毒性影响的热带假丝酵母细胞中，NAC与TOC、LA与VC的组合可提高总不饱和脂肪酸、MUFA和PUFA的水平。
