Ristić-Medić Danijela, Ristić Gordana, Tepsić Vesna, Ristić Gordana N
Institute for Medical Research, Laboratory for Nutrition & Metabolism, PO Box 102, Dr Subotica 4a, 11 129, Belgrade, Serbia and Montenegro.
J Nutr Sci Vitaminol (Tokyo). 2003 Dec;49(6):367-74.
The present study investigated the quantitative effect of dietary fats and ingestion of alcohol on serum and liver lipids, fatty acid bound to phospholipids and their class distribution of male Wistar rats. The rats in C (control) and A (alcohol) groups were fed a standard laboratory diet, HFC (high fat-control) and HFA (high fat-alcohol) groups were fed a high fat diet (standard diet supplemented with 20 g%w/w, sunflower oil: lard mixture 1: 1) for 6 wk. Alcohol-treated rats consumed alcohol at the rate of 9 g/kgbw/d (15-20% energy). Liver phospholipid (PL) content was decreased, and phospholipid/cholesterol liver molar ratio increased in the alcohol treated rats. The proportion of serum sphingophospholipid (Sph) was significantly lower and proportion of phosphatidylcholin (PC) significantly higher in serum PL in alcohol-treated rats. Phospholipid class distribution was unaffected by alcohol feeding in liver. Significantly lower levels of 16:1n-7 and higher levels of 20:5n-3 and 22:4n-6 in the serum PL were observed in the alcohol-treated rats. The groups on the HF diet increased levels of 20:4n-6, 22:4n-6 and total n-6, polyunsaturated fatty acid (PUFA) and decreased levels of 18:1n-9 and total monounsaturated fatty acids (MUFA)in both liver and serum PL, but n-3 fatty acid increased in serum PL and decreased in liver PL compared to groups on the standard diet. Alcohol fat interaction was evident in MUFA and PUFA/SFA in serum PL and n-6, MUFA, PUFA and polyunsaturated/saturated fatty acid ratios (PUFA/SFA) in liver PL. This study showed that the high fat intake in alcohol-treated rats increased levels of 20:4n-6, 22:4n-6 and 20:4/18:2 ratio, and decreased level of 18:1n-9 in liver and serum phospholipids.
本研究调查了膳食脂肪和酒精摄入对雄性Wistar大鼠血清和肝脏脂质、与磷脂结合的脂肪酸及其类别分布的定量影响。C（对照）组和A（酒精）组的大鼠喂食标准实验室饮食，HFC（高脂肪对照）组和HFA（高脂肪酒精）组喂食高脂肪饮食（标准饮食补充20 g%w/w的向日葵油与猪油1:1混合物），持续6周。经酒精处理的大鼠以9 g/kgbw/d的速率摄入酒精（占能量的15 - 20%）。经酒精处理的大鼠肝脏磷脂（PL）含量降低，肝脏磷脂/胆固醇摩尔比增加。经酒精处理的大鼠血清PL中鞘磷脂（Sph）比例显著降低，磷脂酰胆碱（PC）比例显著升高。肝脏中磷脂类别分布不受酒精喂养影响。在经酒精处理的大鼠血清PL中观察到16:1n - 7水平显著降低，20:5n - 3和22:4n - 6水平升高。高脂肪饮食组的肝脏和血清PL中20:4n - 6、22:4n - 6和总n - 6多不饱和脂肪酸（PUFA）水平升高，18:1n - 9和总单不饱和脂肪酸（MUFA）水平降低，但与标准饮食组相比，血清PL中n - 3脂肪酸增加，肝脏PL中n - 3脂肪酸减少。血清PL中的MUFA以及PUFA/SFA和肝脏PL中的n - 6、MUFA、PUFA以及多不饱和/饱和脂肪酸比值（PUFA/SFA）存在酒精与脂肪的相互作用。本研究表明，经酒精处理的大鼠高脂肪摄入会增加肝脏和血清磷脂中20:4n - 6、22:4n - 6水平以及20:4/18:2比值，并降低18:1n - 9水平。
