[维甲酸等诱导大鼠骨髓间充质干细胞向神经元样细胞的体外分化]

[In vitro differentiation of rat bone mesenchymal stem cells into neuron-like cells induced by vitamin A acid etc].

作者信息

Tang Yun'an, Wang Ruishu, Zhang Cheng, Liu Yuqing, Xu Peiyu, Huang Hui

机构信息

Department of Toxicology, West China School of Public Health, Sichuan University, Chengdu 610041, China.

出版信息

Sichuan Da Xue Xue Bao Yi Xue Ban. 2003 Jul;34(3):377-80.

PMID:12910667

Abstract

OBJECTIVE

To explore the feasibility of inducing rat bone mesenchymal stem cells (BMSC) to differentiate into neuron-like cells with the use of Vitamin A acid, zinc and rat injured spinal cord extracts in vitro.

METHODS

The BMSC were isolated from rat, cultured for 4 passages, and were treated with 10 ng/ml basic fibroblast growth factor (bFGF) for 24 h before induction. Then the medium was replaced by an induction media containing Vitamin A acid, zinc and rat injured spinal cord extracts. The morphological changes of the cells were observed. At day 12 of induction, the cells were stained immunocytochemically with neuron-specific enolase (NSE), neurofilament (NF) and glial fibrillary acidic protein (GFAP) antibodies.

RESULTS

At day 12 of induction, a certain number of BMSC became neuron-like cells and showed NSE and NF expression. But the neuron-like cells did not express GFAP.

CONCLUSION

The BMSC can be induced to differentiate into neuron-like cells with the use of Vitamin A acid, zinc and rat injured spinal cord extracts.

摘要

目的

探讨在体外利用维甲酸、锌及大鼠损伤脊髓提取物诱导大鼠骨髓间充质干细胞（BMSC）分化为神经元样细胞的可行性。

方法

从大鼠分离BMSC，培养4代，诱导前用10 ng/ml碱性成纤维细胞生长因子（bFGF）处理24小时。然后将培养基换成含有维甲酸、锌及大鼠损伤脊髓提取物的诱导培养基。观察细胞的形态变化。诱导第12天时，用神经元特异性烯醇化酶（NSE）、神经丝（NF）和胶质纤维酸性蛋白（GFAP）抗体对细胞进行免疫细胞化学染色。