Tang Yun'an, Wang Ruishu, Zhang Cheng, Liu Yuqing, Xu Peiyu, Huang Hui
Department of Toxicology, West China School of Public Health, Sichuan University, Chengdu 610041, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2003 Jul;34(3):377-80.
To explore the feasibility of inducing rat bone mesenchymal stem cells (BMSC) to differentiate into neuron-like cells with the use of Vitamin A acid, zinc and rat injured spinal cord extracts in vitro.
The BMSC were isolated from rat, cultured for 4 passages, and were treated with 10 ng/ml basic fibroblast growth factor (bFGF) for 24 h before induction. Then the medium was replaced by an induction media containing Vitamin A acid, zinc and rat injured spinal cord extracts. The morphological changes of the cells were observed. At day 12 of induction, the cells were stained immunocytochemically with neuron-specific enolase (NSE), neurofilament (NF) and glial fibrillary acidic protein (GFAP) antibodies.
At day 12 of induction, a certain number of BMSC became neuron-like cells and showed NSE and NF expression. But the neuron-like cells did not express GFAP.
The BMSC can be induced to differentiate into neuron-like cells with the use of Vitamin A acid, zinc and rat injured spinal cord extracts.
探讨在体外利用维甲酸、锌及大鼠损伤脊髓提取物诱导大鼠骨髓间充质干细胞（BMSC）分化为神经元样细胞的可行性。
从大鼠分离BMSC，培养4代，诱导前用10 ng/ml碱性成纤维细胞生长因子（bFGF）处理24小时。然后将培养基换成含有维甲酸、锌及大鼠损伤脊髓提取物的诱导培养基。观察细胞的形态变化。诱导第12天时，用神经元特异性烯醇化酶（NSE）、神经丝（NF）和胶质纤维酸性蛋白（GFAP）抗体对细胞进行免疫细胞化学染色。
诱导第12天时，一定数量的BMSC变成神经元样细胞并显示NSE和NF表达。但神经元样细胞不表达GFAP。
利用维甲酸、锌及大鼠损伤脊髓提取物可诱导BMSC分化为神经元样细胞。
