[大鼠骨髓基质细胞向神经元样细胞的分化]
[Differentiation of rat bone marrow stromal cells into neuron like cells].
作者信息
Li Chang-Qi, Liu Dan, Wu Xiao-Qiong
机构信息
Department of Human Anatomy and Neurobiology, Xiangya School of Medicine, Central South University, Changsha 410078, China.
出版信息
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2004 Feb;29(1):18-20.
OBJECTIVE
To investigate the differentiation of the rat bone marrow stromal cells (BMSCs) into neuron like cells which express glutamic acid decarboxylase (GAD) and choline acetyltransferase (ChAT).
METHODS
BMSCs of rats were flushed out with DMEM from femurs marrow and separated, and then planted in plastic flask. After being expanded and purified, the BMSCs were differentiated into neuron-like cells. Neurofilament (NF-200), microassociate protein-2 (MAP-2), neuron-specific nuclear protein (NeuN), nestin, gial fibrillary acidic protein (GFAP), GAD and ChAT were detected by immunohisitochemistry.
RESULTS
The induced BMSCs exhibited neuronal morphological characteristics, and the expressions of NF-200, MAP-2, NeuN and nestin were positive, but the expression of GFAP was negative. About (3 +/- 0.8)% of the induced BMSCs expressed GAD, and (5 +/- 0.3)% expressed ChAT.
CONCLUSION
BMSCs can differentiate into neuron like cells, and express GAD and ChAT partially.
目的
研究大鼠骨髓基质细胞（BMSCs）向表达谷氨酸脱羧酶（GAD）和胆碱乙酰转移酶（ChAT）的神经元样细胞的分化情况。
方法
用DMEM从大鼠股骨骨髓中冲出BMSCs并进行分离，然后接种于塑料培养瓶中。在扩增和纯化后，将BMSCs分化为神经元样细胞。通过免疫组织化学检测神经丝（NF-200）、微管相关蛋白-2（MAP-2）、神经元特异性核蛋白（NeuN）、巢蛋白、胶质纤维酸性蛋白（GFAP）、GAD和ChAT。
结果
诱导后的BMSCs呈现神经元形态特征，NF-200、MAP-2、NeuN和巢蛋白的表达呈阳性，而GFAP的表达呈阴性。约（3±0.8）%的诱导BMSCs表达GAD，（5±0.3）%表达ChAT。
结论
BMSCs可分化为神经元样细胞，并部分表达GAD和ChAT。
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