Yu Bo, Yao Yujia, Yuan Lixing, Li Weiru, Yang Fan
Department of Pediatricus, West China Second Hospital, Sichuan University, Chengud 610041, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2003 Jul;34(3):445-8.
By investigating the changes of filamin distribution in renal tubular epithelial cells at different ischemia/reperfusion time stages we studied the role of filamin in kidney ischemia/reperfusion injury.
The neonatal SD rat ischemia/reperfusion model was set up. Immunofluorescent staining was used to show the distribution of filamin in renal tubular epithelial cells and the quantities of filamin were counted by ImagePlus-Pro system.
Filamin was located around the basal plasma membrane in normal renal tubular epithelial cells. After ischemia for 0.5 h, the redistribution was not significant. After reperfusion for 0.5 h, filamin came into the cell plasma, appeared at the apex of cells and in the lumen. This kind of change was most apparent after reperfusion for 2 h and accompanied with destruction of renal tubules. The regeneration started 24 h post-ischemia. Filamin was redistributed to the basal plasma membrane. After reperfusion for 120 h, the regeneration ended and the structure of renal tubules became normal.
The distribution of filamin was changed during ischemia/reperfusion injury, this change was prior to the change of actin cytoskeleton and integrin.
通过研究不同缺血/再灌注时间阶段肾小管上皮细胞中细丝蛋白分布的变化，探讨细丝蛋白在肾脏缺血/再灌注损伤中的作用。
建立新生SD大鼠缺血/再灌注模型。采用免疫荧光染色显示肾小管上皮细胞中细丝蛋白的分布，并通过ImagePlus-Pro系统对细丝蛋白的数量进行计数。
在正常肾小管上皮细胞中，细丝蛋白位于基底质膜周围。缺血0.5小时后，重新分布不明显。再灌注0.5小时后，细丝蛋白进入细胞质，出现在细胞顶端和管腔中。这种变化在再灌注2小时后最为明显，并伴有肾小管破坏。缺血24小时后开始再生。细丝蛋白重新分布到基底质膜。再灌注120小时后，再生结束，肾小管结构恢复正常。
在缺血/再灌注损伤过程中细丝蛋白分布发生改变，这种改变先于肌动蛋白细胞骨架和整合素的改变。
