Hao Yuman, Luo Zuming, Zhou Dong, Gao Li
Department of Neurology, West China Hospital, Sichuan University, Chengdu, 610041, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2003 Jul;34(3):455-8.
To investigate the ischemic tolerance induced by focal ischemic preconditioning (PC) in rats.
Ten minutes of middle cerebral artery occlusion(MCAO) was used for PC. Different duration of reperfusion (1, 3, 7, 14, 21 and 28 days) was allowed before 2 hours of MCAO followed by 22 hours of reperfusion in SD rats that were divided into the PC group and the PC plus MCAO group, and were compared with the rats of sham-operation plus MCAO group. Neurological scores, infarct volume, brain water content and HE staining were evaluated in each group.
PC produced no neurological deficits and TTC-demonstrated infarct. PC reduced neurological deficits significantly caused by 2 h MCAO 1-28 days after PC. Infarct volume was reduced only if PC was performed 1-14 days before MCAO. PC given 3 days before MCAO also decreased brain water content.
Ten minutes of MCAO was strong enough to induce ischemic tolerance without brain injury. Infarct volume reduction appeared at 1 day and did not vanish until 14 days after PC, but protection against neurological impairment existed at least 4 weeks.
研究局灶性缺血预处理（PC）诱导大鼠产生缺血耐受的情况。
采用大脑中动脉闭塞（MCAO）10分钟进行PC。在SD大鼠中，PC组和PC加MCAO组在MCAO 2小时前分别给予不同时长的再灌注（1、3、7、14、21和28天），随后再灌注22小时，并与假手术加MCAO组大鼠进行比较。评估每组的神经功能评分、梗死体积、脑含水量及HE染色情况。
PC未产生神经功能缺损及TTC显示的梗死。PC后1 - 28天，PC可显著减轻由2小时MCAO所致的神经功能缺损。仅在MCAO前1 - 14天进行PC可使梗死体积减小。MCAO前3天给予PC也可降低脑含水量。
10分钟的MCAO足以诱导缺血耐受而不造成脑损伤。梗死体积减小出现在PC后1天，直至14天后才消失，但对神经功能损害的保护作用至少持续4周。
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