[儿童急性白血病中hTERT基因表达的研究]
[Studies of hTERT gene expression in acute leukemia children].
作者信息
Li Xihong, Wang Xiaoyang, Li Xinxiao, Liu Bailin, Zhang Yan, Liao Qingkui
机构信息
Department of Pediatrics, West China Second Hospital, Sichuan University, Chengdu 610041, China.
出版信息
Sichuan Da Xue Xue Bao Yi Xue Ban. 2003 Jul;34(3):486-8.
OBJECTIVE
To explore the gene expression of human telomerase reverse transcriptase (hTERT) in acute leukemia (AL) children and its clinical implication.
METHODS
Expression of hTERT mRNA was detected in K562 leukemia cell line, 40 acute leukemia children (AL) and 10 healthy subjects by reverse transcriptase-polymerase chain reaction (RT-PCR).
RESULTS
hTERT gene was expressed in K562 cell line, acute lymphoblastic leukemia children (23/30), acute myeloid leukemia children (7/10), and healthy subject (1/10). The expression level of hTERT gene was observed in all subtypes of AL and was related to the prognosis of AL, and the patients with relative level > or = 1.0 were found to have lower complete remission (CR) rates and disease-free survival.
CONCLUSION
hTERT gene expression is associated with the pathogenesis of childhood AL. It can serve as a prognostic indicator in childhood AL.
目的
探讨人端粒酶逆转录酶（hTERT）在急性白血病（AL）患儿中的基因表达及其临床意义。
方法
采用逆转录聚合酶链反应（RT-PCR）检测K562白血病细胞系、40例急性白血病患儿（AL）及10例健康对照者中hTERT mRNA的表达。
结果
hTERT基因在K562细胞系、急性淋巴细胞白血病患儿（23/30）、急性髓细胞白血病患儿（7/10）及健康对照者（1/10）中均有表达。hTERT基因在AL各亚型中均有表达，且与AL的预后相关，相对水平≥1.0的患者完全缓解（CR）率及无病生存率较低。
结论
hTERT基因表达与儿童AL的发病机制有关，可作为儿童AL的预后指标。
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