Horsztyński Dariusz, Wawer Iwona, Grieb Paweł
Kliniki Neurochirurgii Instytutu Medycyny Doświadczalnej i Klinicznej PAN w Warszawie.
Neurol Neurochir Pol. 2003 Jan-Feb;37(1):123-32.
Proton magnetic spectroscopy of brain tumor extracts, and more recently magic angle spinning proton magnetic resonance spectroscopy (MAS 1H-MRS) of intact cooled tissue specimens have been used for tumor classification. The aim of this study was to obtain MAS 1H-MRS spectra of lyophilised and pulverised specimens of various brain tumors and to assess their utility for tumor type recognition.
Tissue samples of intracranial tumors were obtained at surgery and frozen immediately in liquid nitrogen. A part of each sample was subjected to a routine histopathological examination, while the rest was lyophilised, pulverised, and subjected to high-speed magic angle spinning MRS using Bruker DSX-400 WB AVANCE spectrometer and rotation speed up to 33 kHz.
MAS 1H-MRS spectra of 39 samples (including 16 glioblastomas or gliosarcomas, 10 meningiomas and 5 craniopharyngiomas) were obtained. Invariably the spectra were dominated by broad lipid resonances, reflecting "NMR visible" protons in methyl,methylene and methine groups of fatty acid chains. Resolution of the spectra was markedly higher for glioblastomas/gliosarcomas and craniopharyngiomas than for meningiomas.
Lyophylization of brain tumor specimens eliminates signals of watersoluble metabolites and results in a loss of discriminative power of the 1H-MRS analysis. Resolution of the spectra is increased for tumors that accumulate lipids extracellularly, but is not related to tumor histological grade.
脑肿瘤提取物的质子磁共振波谱，以及最近对完整的冷冻组织标本进行的魔角旋转质子磁共振波谱（MAS 1H-MRS）已用于肿瘤分类。本研究的目的是获取各种脑肿瘤冻干和粉碎标本的MAS 1H-MRS波谱，并评估其在肿瘤类型识别中的效用。
在手术中获取颅内肿瘤组织样本，并立即在液氮中冷冻。每个样本的一部分进行常规组织病理学检查，其余部分进行冻干、粉碎，并使用布鲁克DSX-400 WB AVANCE光谱仪和高达33 kHz的转速进行高速魔角旋转MRS。
获得了39个样本的MAS 1H-MRS波谱（包括16个胶质母细胞瘤或胶质肉瘤、10个脑膜瘤和5个颅咽管瘤）。这些波谱总是以宽脂质共振为主，反映了脂肪酸链的甲基、亚甲基和次甲基基团中的“NMR可见”质子。胶质母细胞瘤/胶质肉瘤和颅咽管瘤的波谱分辨率明显高于脑膜瘤。
脑肿瘤标本的冻干消除了水溶性代谢物的信号，并导致1H-MRS分析的鉴别能力丧失。对于细胞外积累脂质的肿瘤，波谱分辨率增加，但与肿瘤组织学分级无关。
