Barton S J, Howe F A, Tomlins A M, Cudlip S A, Nicholson J K, Bell B A, Griffiths J R
CRC Biomedical Magnetic Resonance Research Group, Division of Biochemistry, St. George's Hospital Medical School, London, UK.
MAGMA. 1999 May;8(2):121-8. doi: 10.1007/BF02590529.
High resolution magic angle spinning (MAS) 1H nuclear magnetic resonance (NMR) spectroscopy has been employed to study intact human brain tumour tissue and comparison with the corresponding in vivo spectrum has been made. Two dimensional 1H MAS-NMR measurements, including J-resolved and homonuclear shift correlation spectra, were obtained to aid metabolite signal assignment. MAS gave greatly improved line-shape and reduced line-width in comparison to conventional high resolution in vivo 1H MRS of intact tissue, permitting the simultaneous detection of cellular lipids and metabolites. The technique provides the most direct method for comparison of in vivo spectra with high resolution spectra in vitro and hence allows more reliable peak assignment of in vivo 1H MRS spectra.
高分辨率魔角旋转（MAS）1H核磁共振（NMR）光谱已被用于研究完整的人脑肿瘤组织，并与相应的体内光谱进行了比较。获得了二维1H MAS-NMR测量结果，包括J分辨和同核位移相关光谱，以辅助代谢物信号归属。与完整组织的传统高分辨率体内1H磁共振波谱相比，MAS极大地改善了线形并减小了线宽，从而能够同时检测细胞脂质和代谢物。该技术为体内光谱与体外高分辨率光谱的比较提供了最直接的方法，因此能够更可靠地对体内1H MRS光谱进行峰归属。
