Goumenos Dimitrios S, Tsakas Sotiris, Karavias Dionisios, Savidaki Irini, Karatzas Thoedoros, Vlachojannis John G
Department of Internal Medicine-Nephrology, University Hospital, Patras, Greece.
Ren Fail. 2003 Jul;25(4):561-8. doi: 10.1081/jdi-120022547.
Transforming growth factor-beta1 (TGF-beta1), the major fibrogenic growth factor, is implicated in the pathogenesis of renal scarring in experimental and clinical nephropathies as well as in chronic allograft nephropathy. In this study we examined the pattern of changes of TGF-beta1 excretion in the urine and the sites of TGF-beta1 expression in the kidney of transplanted patients during the early post-transplantation period.
Eighteen renal allograft recipients were included in the study. In all patients urinary TGF-beta1 levels were determined by ELISA in sequential measurements during the first two postoperative months and compared to that of 14 healthy subjects. The renal expression of TGF-beta1 protein was studied in 4 patients that underwent a biopsy of the transplanted kidney at the same period. All patients were treated with prednisolone, cyclosporin, and mycophenolate mofetil.
Urinary TGF-beta1 levels were increased during the first postoperative days. Although they were gradually reduced during the first two post-operative months, they remained significantly higher compared to those of normal subjects (580 +/- 148 ng/24 h vs. 310 +/- 140 ng/ 24 h p < 0.01). The decline of urinary TGF-beta1 excretion followed that of serum creatinine. TGF-beta1 protein expression was identified within the cytoplasm of tubular epithelial cells of transplanted patients.
Elevated urinary TGF-beta1 levels are observed during the early post-transplantation period in renal allograft recipients and are maintained high even after restoration of renal function to normal.
转化生长因子-β1（TGF-β1）是主要的促纤维化生长因子，在实验性和临床肾病以及慢性移植肾肾病的肾瘢痕形成发病机制中发挥作用。在本研究中，我们检测了移植患者术后早期尿液中TGF-β1排泄的变化模式以及肾脏中TGF-β1的表达部位。
本研究纳入了18例肾移植受者。所有患者在术后的头两个月内通过ELISA连续测定尿TGF-β1水平，并与14名健康受试者的水平进行比较。同期对4例接受移植肾活检的患者研究了TGF-β1蛋白在肾脏中的表达。所有患者均接受泼尼松龙、环孢素和霉酚酸酯治疗。
术后最初几天尿TGF-β1水平升高。尽管在术后的头两个月内逐渐降低，但仍显著高于正常受试者（580±148 ng/24 h对310±140 ng/24 h，p<0.01）。尿TGF-β1排泄量的下降与血清肌酐的下降一致。在移植患者肾小管上皮细胞的细胞质中鉴定到了TGF-β1蛋白表达。
肾移植受者在术后早期尿TGF-β1水平升高，即使肾功能恢复正常后仍维持在较高水平。
