黏膜内结肠肿瘤中的γ-氨基丁酸免疫反应性

Gamma-amino-butyric acid immunoreactivity in intramucosal colonic tumors.

作者信息

Maemura Kentaro, Yamauchi Hiroaki, Hayasaki Hana, Kanbara Kiyoto, Tamayama Takumi, Hirata Ichiro, Watanabe Masahito

机构信息

Department of Anatomy, Osaka Medical College, 2-7 Daigaku-machi, Takatsuki, Osaka 569-8686, Japan.

出版信息

J Gastroenterol Hepatol. 2003 Sep;18(9):1089-94. doi: 10.1046/j.1440-1746.2003.03131.x.

DOI:10.1046/j.1440-1746.2003.03131.x

PMID:12911668

Abstract

BACKGROUND AND AIM

The level of gamma-amino-butyric acid (GABA) is reported to be increased in colon cancer. Moreover, data suggests that GABA plays a role in the proliferation or maturation of some types of cells. We examined the expression of GABA in intramucosal colonic tumors to clarify the relation between GABA and the degree of atypia.

METHODS

Paraffin sections were prepared from 56 protruded-type colonic neoplasms, which were classified as intramucosal adenocarcinoma (AC), adenoma with severe atypia (ASA), or adenoma with mild to moderate atypia (AMA). Expression of GABA was investigated immunohistochemically, and GABA immunoreactivity was compared to the staining patterns of carcinoembryonic antigen (CEA) and cancer-associated antigen (CA19-9) which were classified into three categories.

RESULTS

Intense GABA immunoreactivity was observed in 73.7%, 54.6%, 13.3%, and 5.4% of AC, ASA, AMA, and normal mucosa specimens, respectively. Kendall's correlation coefficient between GABA immunoreactivity and the degree of atypia was 0.447. Strong, positive CEA staining (pattern 3) was observed in 57.9%, 36.3%, and 13.3% of AC, ASA, and AMA specimens, respectively. Strong, positive CA19-9 staining was observed: 26.3%, 9.1% and 0%, respectively. In AC and ASA, the proportion of glands with strong GABA immunoreactivity was greater than the proportion of glands that were strongly positive for CA19-9.

CONCLUSION

GABA may be useful as a tumor marker in combination with other tumor markers such as CEA and CA19-9.

摘要

背景与目的

据报道，结肠癌中γ-氨基丁酸（GABA）水平会升高。此外，数据表明GABA在某些类型细胞的增殖或成熟过程中发挥作用。我们检测了黏膜内结肠肿瘤中GABA的表达，以阐明GABA与异型程度之间的关系。

方法

从56例突出型结肠肿瘤制备石蜡切片，这些肿瘤被分类为黏膜内腺癌（AC）、重度异型腺瘤（ASA）或轻至中度异型腺瘤（AMA）。采用免疫组织化学方法研究GABA的表达，并将GABA免疫反应性与癌胚抗原（CEA）和癌相关抗原（CA19-9）的染色模式进行比较，后者分为三类。

结果

分别在73.7%、54.6%、13.3%和5.4%的AC、ASA、AMA和正常黏膜标本中观察到强烈的GABA免疫反应性。GABA免疫反应性与异型程度之间的肯德尔相关系数为0.447。分别在57.9%、36.3%和13.3%的AC、ASA和AMA标本中观察到强阳性CEA染色（模式3）。观察到强阳性CA19-9染色的比例分别为26.3%、9.1%和0%。在AC和ASA中，具有强GABA免疫反应性的腺体比例大于对CA19-9呈强阳性的腺体比例。