Bréhélin Claire, Meyer Etienne H, de Souris Jean-Paul, Bonnard Géraldine, Meyer Yves
Laboratoire Génome et Développement des Plantes, Université de Perpignan, Unité Mixte de Recherche Centre National de la Recherche Scientifique 5096, 52 avenue de Villeneuve, 66860 Perpignan, France.
Plant Physiol. 2003 Aug;132(4):2045-57. doi: 10.1104/pp.103.022533.
The Arabidopsis type II peroxiredoxin (PRXII) family is composed of six different genes, five of which are expressed. On the basis of the nucleotide and protein sequences, we were able to define three subgroups among the PRXII family. The first subgroup is composed of AtPRXII-B, -C, and -D, which are highly similar and localized in the cytosol. AtPRXII-B is ubiquitously expressed. More striking is the specific expression of AtPRXII-C and AtPRXII-D localized in pollen. The second subgroup comprises the mitochondrial AtPRXII-F, the corresponding gene of which is expressed constitutively. We show that AtPRXII-E, belonging to the last subgroup, is expressed mostly in reproductive tissues and that its product is addressed to the plastid. By in vitro enzymatic experiments, we demonstrate that glutaredoxin is the electron donor of recombinant AtPRXII-B for peroxidase reaction, but the donors of AtPRXII-E and AtPRXII-F have still to be identified.
拟南芥II型过氧化物酶体蛋白（PRXII）家族由六个不同的基因组成，其中五个基因表达。根据核苷酸和蛋白质序列，我们能够在PRXII家族中定义三个亚组。第一个亚组由AtPRXII-B、-C和-D组成，它们高度相似，定位于细胞质中。AtPRXII-B在各处均有表达。更引人注目的是，AtPRXII-C和AtPRXII-D特异性表达于花粉中。第二个亚组包括线粒体AtPRXII-F，其相应基因持续表达。我们表明，属于最后一个亚组的AtPRXII-E主要在生殖组织中表达，其产物定位于质体。通过体外酶实验，我们证明谷氧还蛋白是重组AtPRXII-B过氧化物酶反应的电子供体，但AtPRXII-E和AtPRXII-F的供体仍有待确定。
