Dombrowski James E
U.S. Department of Agriculture-Agricultural Research Service, National Forage Seed Production Research Center, Oregon State University, Corvallis, Oregon 97331-7102, USA.
Plant Physiol. 2003 Aug;132(4):2098-107. doi: 10.1104/pp.102.019927.
Plants respond to various stresses by expressing distinct sets of genes. The effects of multiple stresses on plants and their interactions are not well understood. We have discovered that salt stress causes the accumulation of proteinase inhibitors and the activation of other wound-related genes in tomato (Lycopersicon esculentum) plants. Salt stress was also found to enhance the plant's response to wounding locally and systemically. The tomato mutant (def-1), which has an impairment in the octadecanoid pathway, displayed a severe reduction in the accumulation of proteinase inhibitors under salt stress, indicating that salt stress-induced accumulation of proteinase inhibitors was jasmonic acid dependent. The analysis of salt stress in another tomato mutant, spr-1, which carries a mutation in a systemin-specific signaling component, and transgenic tomato plants that express an antisense-prosystemin cDNA, showed that prosystemin activity was not required for the salt-induced accumulation of proteinase inhibitors, but was necessary to achieve maximal levels. These results suggest that a prosystemin independent- but jasmonic acid-dependent pathway is utilized for proteinase inhibitor accumulation in response to salt stress.
植物通过表达不同的基因集来应对各种胁迫。多种胁迫对植物的影响及其相互作用尚未得到充分了解。我们发现盐胁迫会导致番茄（Lycopersicon esculentum）植株中蛋白酶抑制剂的积累以及其他与伤口相关基因的激活。还发现盐胁迫会增强植物对局部和全身伤口的反应。番茄突变体（def-1）在十八烷途径中存在缺陷，在盐胁迫下蛋白酶抑制剂的积累严重减少，这表明盐胁迫诱导的蛋白酶抑制剂积累依赖于茉莉酸。对另一个番茄突变体spr-1（其在系统素特异性信号成分中发生突变）以及表达反义前系统素cDNA的转基因番茄植株的盐胁迫分析表明，盐诱导的蛋白酶抑制剂积累不需要前系统素活性，但达到最大水平是必需的。这些结果表明，在响应盐胁迫时，蛋白酶抑制剂积累利用了一条不依赖前系统素但依赖茉莉酸的途径。
