Natale Roberto, Coppola Mariangela, D'Agostino Nunzio, Zhang Youjun, Fernie Alisdair Robert, Castaldi Valeria, Rao Rosa
Department of Agricultural Sciences, University of Naples Federico II, Portici 80055, Italy.
Max-Planck-Institut für Molekulare Pflanzenphysiologie, Potsdam-Golm 14476, Germany.
Comput Struct Biotechnol J. 2022 Dec 7;21:212-223. doi: 10.1016/j.csbj.2022.12.006. eCollection 2023.
Tomato Prosystemin (ProSys), the precursor of Systemin, a small peptidic hormone, is produced at very low concentration in unchallenged plants, while its expression greatly increases in response to several different stressors triggering an array of defence responses. The molecular mechanisms that underpin such a wide array of defence barriers are not fully understood and are likely correlated with the intrinsically disordered (ID) structure of the protein. ID proteins interact with different protein partners forming complexes involved in the modulation of different biological mechanisms. Here we describe the ProSys-protein network that shed light on the molecular mechanisms underpinning ProSys associated defence responses. Three different approaches were used. prediction resulted in 98 direct interactors, most clustering in phytohormone biosynthesis, transcription factors and signal transduction gene classes. The network shows the central role of ProSys during defence responses, that reflects its role as central hub. ProSys interactors, identified by Affinity Purification-Mass Spectrometry (AP-MS), revealed over three hundred protein partners, while Bimolecular Fluorescent Complementation (BiFC) experiments validated some interactors predicted and . Our results demonstrate that ProSys interacts with several proteins and reveal new key molecular events in the ProSys-dependent defence response of tomato plant.
番茄原系统素（ProSys）是系统素（一种小肽激素）的前体，在未受胁迫的植物中以极低的浓度产生，而在对几种不同胁迫因子作出反应时其表达会大幅增加，从而引发一系列防御反应。支撑如此众多防御屏障的分子机制尚未完全了解，并且可能与该蛋白质的内在无序（ID）结构相关。ID蛋白与不同的蛋白质伙伴相互作用，形成参与调节不同生物学机制的复合物。在这里，我们描述了ProSys-蛋白质网络，该网络揭示了支撑ProSys相关防御反应的分子机制。我们使用了三种不同的方法。预测产生了98个直接相互作用分子，大多数聚集在植物激素生物合成、转录因子和信号转导基因类别中。该网络显示了ProSys在防御反应中的核心作用，这反映了它作为中心枢纽的角色。通过亲和纯化-质谱（AP-MS）鉴定的ProSys相互作用分子揭示了三百多个蛋白质伙伴，而双分子荧光互补（BiFC）实验验证了一些预测的相互作用分子。我们的结果表明，ProSys与多种蛋白质相互作用，并揭示了番茄植株ProSys依赖性防御反应中的新关键分子事件。
