[抑制化学发光法测定抗坏血酸]

[Determination of ascorbic acid by suppressed chemiluminescence].

作者信息

Ma Quan-li, Jin Yong-ping, You Hong-tao, Ma Hui-min

机构信息

Center for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China.

出版信息

Guang Pu Xue Yu Guang Pu Fen Xi. 2002 Dec;22(6):888-90.

PMID:12914155

Abstract

This paper describes a method for determining ascorbic acid by the suppression of DTMC-H2O2 chemiluminescence. Various factors affecting the chemiluminescence determination of ascorbic acid were examined. Under the optimal conditions (1.2 x 10(-4) mol.L-1 of DTMC, 5.0 x 10(-5) mol.L-1 of H2O2, and pH 11.4 Na2HPO4-NaOH), the quenching of the chemiluminescent intensity was proportional to the concentration of ascorbic acid in the range of 1.0 x 10(-7)-8.0 x 10(-6) mol.L-1, and the detection limit was 8.0 x 10(-8) mol.L-1 (S/N = 3). The RSD was 4.6% for 1.0 x 10(-6) mol.L-1 of ascorbic acid (n = 10). The selectivity of this method was high and no making agent required. The proposed method has been applied to the determination of ascorbic acid in real samples. The obtained results were in agreement with those by CuSO4-KI titration.

摘要

本文描述了一种通过抑制DTMC-H2O2化学发光来测定抗坏血酸的方法。研究了影响抗坏血酸化学发光测定的各种因素。在最佳条件下（1.2×10(-4) mol·L-1的DTMC、5.0×10(-5) mol·L-1的H2O2和pH 11.4的Na2HPO4-NaOH），化学发光强度的猝灭与抗坏血酸浓度在1.0×10(-7)-8.0×10(-6) mol·L-1范围内成正比，检测限为8.0×10(-8) mol·L-1（S/N = 3）。对于1.0×10(-6) mol·L-1的抗坏血酸，相对标准偏差为4.6%（n = 10）。该方法选择性高，无需使用掩蔽剂。所提出的方法已应用于实际样品中抗坏血酸的测定。所得结果与硫酸铜-碘化钾滴定法的结果一致。