School of Chemistry and Chemical Engineering, Sun Yat-Sen University, 135 Xingang Xi Road, Guangzhou, Guangdong 510275, China.
Talanta. 2010 Apr 15;81(1-2):499-504. doi: 10.1016/j.talanta.2009.12.032. Epub 2009 Dec 29.
In this paper, a novel flow injection-chemiluminescence (FI-CL) method was proposed for the determination of ergometrine maleate in serum. The new CL reaction was based on the direct oxidation of ergometrine maleate by the complex of metal chelate diperiodatocuprate(III) (K(5)[Cu(HIO(6))(2)]) in an alkaline medium. The CL intensity was enhanced in the presence of ascorbic acid. Hereby under the optimum conditions, ergometrine maleate was determined over the range of 4.0 x 10(-9) gm L(-1) to 4.0 x 10(-7) gm L(-1) with a limit of detection (3 sigma) of 1.1 x 10(-9) gm L(-1). The relative standard deviation (R.S.D.) was 2.1% for 8.0 x 10(-9) gm L(-1) ergometrine maleate (n=7). The sensitive method was successfully applied to the direct determination of ergometrine maleate (ng mL(-1)) in pharmaceutical injection and serum samples. The mechanism of the reactions was also discussed.
本文提出了一种新的流动注射-化学发光（FI-CL）方法，用于测定血清中的马来酸麦角新碱。新的 CL 反应是基于在碱性介质中，金属螯合物高碘酸铜（III）（K（5）[Cu（HIO（6））（2）]）复合物直接氧化马来酸麦角新碱。在抗坏血酸存在下，CL 强度增强。在此最佳条件下，马来酸麦角新碱在 4.0×10（-9）gm L（-1）至 4.0×10（-7）gm L（-1）的范围内进行了测定，检测限（3 倍标准差）为 1.1×10（-9）gm L（-1）。对于 8.0×10（-9）gm L（-1）的马来酸麦角新碱（n=7），相对标准偏差（R.S.D.）为 2.1%。该灵敏方法成功应用于药物注射和血清样品中马来酸麦角新碱（ng mL（-1））的直接测定。还讨论了反应的机制。
