Long Yuejiao, Sun Changhao, Wang Chaoxu
Department of Nutrition and Food Hygiene, Harbin Medical University, Harbin 150001, China.
Wei Sheng Yan Jiu. 2003 May;32(3):209-11.
In order to explore the influences of iron overload on lipid peroxidation of rats and the inhibiting effects of antioxidant vitamins such as vitamin E and beta-carotene on the influences induced by iron overload, eighty male Wistar rats were randomly divided into 8 groups according to the weight. The diets of the former 4 groups differed only in iron concentration (50, 200, 350 or 500 mg/kg diet). The diet of the latter 4 groups were consistent with the former 4 groups respectively, but complemented with vitamin E(100 mg/kg diet) and beta-carotene (25 mg/kg.BW) at the same time. After Eight weeks, the levels of serum iron, blood lipid including total cholesterol TC, triglyceride TG, high density lipoprotein HDL, low density lipoprotein LDL, vitamin E, vitamin A, serum malondialdehyde (MDA) and oxidized low density lipoprotein (OX-LDL), the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were determined. Results showed that the iron overload increased the levels of MDA, OX-LDL and LDL in serum, especially in the group of rat with 500 mg/kg diet. The activity of GSH-Px increased irritably and the contents of Vitamin E and Vitamin A decreased significantly. Complementing antioxidant vitamins decreased the levels of MDA, OX-LDL and LDL and increased HDL and the activity of GSH-Px decreased compared with the corresponding iron overload groups. It was concluded that iron overload could promote lipid peroxidation reaction especially in 10 folds of physiologic dose and the activities of antioxidant enzymes increased at the same time to keep the body homeostasis. Antioxidant vitamins may play a role through non-enzymatic reactive system to inhibit the above changes.
为探讨铁过载对大鼠脂质过氧化的影响以及抗氧化维生素如维生素E和β-胡萝卜素对铁过载所致影响的抑制作用，80只雄性Wistar大鼠按体重随机分为8组。前4组的饮食仅铁浓度不同（分别为50、200、350或500 mg/kg饮食）。后4组的饮食分别与前4组一致，但同时补充维生素E（100 mg/kg饮食）和β-胡萝卜素（25 mg/kg体重）。8周后，测定血清铁、血脂包括总胆固醇（TC）、甘油三酯（TG）、高密度脂蛋白（HDL）、低密度脂蛋白（LDL）、维生素E、维生素A、血清丙二醛（MDA）和氧化型低密度脂蛋白（OX-LDL）水平，以及超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）活性。结果显示，铁过载使血清中MDA、OX-LDL和LDL水平升高，尤其是饮食中铁含量为500 mg/kg的大鼠组。GSH-Px活性急剧升高，维生素E和维生素A含量显著降低。与相应的铁过载组相比，补充抗氧化维生素可降低MDA、OX-LDL和LDL水平，升高HDL水平，且GSH-Px活性降低。结论是，铁过载可促进脂质过氧化反应，尤其是在生理剂量10倍的情况下，同时抗氧化酶活性升高以维持机体稳态。抗氧化维生素可能通过非酶反应系统发挥作用来抑制上述变化。
