Akturk O, Demirin H, Sutcu R, Yilmaz N, Koylu H, Altuntas I
Department of Biochemistry and Clinical Biochemistry, Suleyman Demirel University, School of Medicine, 32260, Isparta, Turkey.
Cell Biol Toxicol. 2006 Nov;22(6):455-61. doi: 10.1007/s10565-006-0138-5. Epub 2006 Sep 11.
Diazinon is one of the most widely used organophosphate insecticides (OPIs) in agriculture and public health programs. Reactive oxygen species (ROS) caused by OPIs may be involved in the toxicity of various pesticides. The aim of this study was to investigate how diazinon affects lipid peroxidation (LPO) and the antioxidant defense system in vivo and the possible ameliorating role of vitamins E and C. For this purpose, experiments were done to study the effects of DI on LPO and the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) in adult rat heart. Experimental groups were: (1) control group, (2) diazinon treated (DI) group, (3) DI+vitamins E and C-treated (DI+Vit) group. The levels of malondialdehyde (MDA) and the activities of SOD and CAT increased significantly in the DI group compared with the control group. The activity of SOD and the levels of MDA decreased significantly in the DI+Vit group compared with the DI group. The differences between the DI+Vit and control groups according to the MDA levels and the activities of both SOD and CAT were statistically significant. These results suggest that treating rats with a single dose of diazinon increases LPO and some antioxidant enzyme activities in the rat myocardium and, in addition, that single-dose treatment with a combination of vitamins E and C after the administration of diazinon can reduce LPO caused by diazinon, though this treatment was not sufficiently effective to reduce the values to those in control group.
二嗪农是农业和公共卫生项目中使用最广泛的有机磷酸酯类杀虫剂之一。有机磷酸酯类杀虫剂产生的活性氧（ROS）可能与多种农药的毒性有关。本研究的目的是探讨二嗪农如何影响体内脂质过氧化（LPO）和抗氧化防御系统，以及维生素E和C可能的改善作用。为此，进行了实验以研究二嗪农对成年大鼠心脏中LPO以及超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）和过氧化氢酶（CAT）活性的影响。实验组包括：（1）对照组，（2）二嗪农处理（DI）组，（3）二嗪农+维生素E和C处理（DI+Vit）组。与对照组相比，DI组中丙二醛（MDA）水平以及SOD和CAT活性显著升高。与DI组相比，DI+Vit组中SOD活性和MDA水平显著降低。根据MDA水平以及SOD和CAT活性，DI+Vit组与对照组之间的差异具有统计学意义。这些结果表明，给大鼠单次注射二嗪农会增加大鼠心肌中的LPO和一些抗氧化酶活性，此外，在注射二嗪农后用维生素E和C联合进行单次剂量治疗可以降低二嗪农引起的LPO，尽管这种治疗不足以将数值降低到对照组的水平。
