Hodgkinson Conrad P, Ye Shu
Human Genetics Division, University of Southampton School of Medicine, Southampton, United Kingdom.
Biochem Biophys Res Commun. 2003 Aug 29;308(3):505-10. doi: 10.1016/s0006-291x(03)01416-5.
We used a combination of expression microarray and Northern blot analyses to identify target genes for peroxisome proliferator-activated receptor (PPAR) gamma in RAW264.7 macrophages. PPARgamma natural ligand 15-deoxy-Delta(12,14) prostaglandin and synthetic ligands ciglitazone and rosiglitazone increased the expression of scavenger receptor CD36 and ATP-binding cassette transporter A1, as well as adipophilin (a lipid droplet coating protein involved in intracellular lipid storage and transport), calpain (a protease implicated in ABCA1 protein degradation), and ADAM8 (a disintegrin and metalloprotease protein involved in cell adhesion). These findings are relevant to understanding the effect of PPARgamma activation on gene expression and cognate pathways in macrophages.
我们运用表达微阵列和Northern印迹分析相结合的方法，在RAW264.7巨噬细胞中鉴定过氧化物酶体增殖物激活受体（PPAR）γ的靶基因。PPARγ天然配体15-脱氧-Δ(12,14)前列腺素以及合成配体环格列酮和罗格列酮可增加清道夫受体CD36和ATP结合盒转运体A1的表达，同时也增加脂肪分化相关蛋白（一种参与细胞内脂质储存和转运的脂滴包被蛋白）、钙蛋白酶（一种与ABCA1蛋白降解有关的蛋白酶）以及ADAM8（一种参与细胞黏附的去整合素和金属蛋白酶蛋白）的表达。这些发现对于理解PPARγ激活对巨噬细胞基因表达及相关通路的影响具有重要意义。
