Department of Endocrinology, Huashan Hospital, Institute of Endocrinology and Diabetology at Fudan University, Fudan University, Shanghai, China.
Mol Cell Biochem. 2010 Apr;337(1-2):193-9. doi: 10.1007/s11010-009-0299-7. Epub 2009 Oct 23.
Macrophages accumulated in the arterial intima play an important role in the development of atherosclerosis by producing a large number of proinflammatory cytokines which accelerate the disease. Recent studies show that adipophilin might be involved in inflammatory processes in macrophages. In this study, we observe the effect of adipophilin on proinflammatory cytokine expression and secretion in THP-1 macrophages. SiRNA and adipophilin gene overexpression mediated by an pEGFP-C3 vector were used to observe the effect of adipophilin on proinflammatory cytokines in THP-1 macrophages in vitro. Realtime PCR and enzyme-linked immunosorbent assay (ELISA) were applied to detect the production of tumor necrosis factor alpha (TNF-alpha), monocyte chemoattractant protein 1 (MCP-1), and interleukin-6 (IL-6). It was found that acetylated low-density lipoprotein (AcLDL), pioglitazone [a peroxisome proliferator-activated receptor gamma (PPARgamma) agonist] increased adipophilin expression in macrophages, while glucose had no such affect. It was also shown that adipophilin augments TNF-alpha, MCP-1, and IL-6 expression in AcLDL induced macrophages. Our results suggest that adipophilin augment inflammation in macrophages, which might be one role of adipophilin in atherosclerosis.
巨噬细胞在动脉内膜中的积累在动脉粥样硬化的发展中起着重要作用，它们产生大量的促炎细胞因子，加速了疾病的进程。最近的研究表明，脂联素可能参与巨噬细胞中的炎症过程。在这项研究中，我们观察了脂联素对 THP-1 巨噬细胞中促炎细胞因子表达和分泌的影响。使用 siRNA 和 pEGFP-C3 载体过表达脂联素，观察脂联素对 THP-1 巨噬细胞中促炎细胞因子的影响。实时 PCR 和酶联免疫吸附试验（ELISA）用于检测肿瘤坏死因子-α（TNF-α）、单核细胞趋化蛋白 1（MCP-1）和白细胞介素-6（IL-6）的产生。结果发现，乙酰化低密度脂蛋白（AcLDL）和吡格列酮[过氧化物酶体增殖物激活受体γ（PPARγ）激动剂]增加了巨噬细胞中的脂联素表达，而葡萄糖则没有这种作用。研究还表明，脂联素增强了 AcLDL 诱导的巨噬细胞中 TNF-α、MCP-1 和 IL-6 的表达。我们的结果表明，脂联素增强了巨噬细胞中的炎症反应，这可能是脂联素在动脉粥样硬化中的作用之一。
