Verdier Laurent, Sakhaii Peyman, Zweckstetter Markus, Griesinger Christian
Max Planck Institute for Biophysical Chemistry, Abt. 030, Am Fassberg 11, 37077 Göttingen, Germany.
J Magn Reson. 2003 Aug;163(2):353-9. doi: 10.1016/s1090-7807(03)00063-6.
In this paper we show that water insoluble compounds dissolved in poly-gamma-benzyl-glutamate are amenable to the measurement of a number of homo- and heteronuclear dipolar couplings. The sensitivity and experimental precision of dipolar couplings are sufficient to obtain a good match with the structure. In order to achieve the necessary precision for H,C dipolar couplings between protons and carbons that are not directly bound a new method for the measurement of heteronuclear long range couplings is introduced that allows a one-parameter fit to a HSQC-based experiment as reference experiment. The methodology is applied to menthol (1R, 3S, 4R).
在本文中，我们表明溶解于聚-γ-苄基谷氨酸中的水不溶性化合物适用于多种同核和异核偶极耦合的测量。偶极耦合的灵敏度和实验精度足以与结构实现良好匹配。为了获得质子与非直接键合碳之间的H,C偶极耦合所需的精度，引入了一种测量异核远程耦合的新方法，该方法允许对基于HSQC的实验进行单参数拟合作为参考实验。该方法应用于薄荷醇（1R, 3S, 4R）。
