用于测量蛋白质中一键13C'-13Cα和13Cα-1Hα剩余偶极耦合的灵敏度增强IPAP实验。

Sensitivity-enhanced IPAP experiments for measuring one-bond 13C'-13Calpha and 13Calpha-1Halpha residual dipolar couplings in proteins.

作者信息

Ding Keyang, Gronenborn Angela M

机构信息

Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA.

出版信息

J Magn Reson. 2004 Apr;167(2):253-8. doi: 10.1016/j.jmr.2003.12.016.

DOI:10.1016/j.jmr.2003.12.016

PMID:15040980

Abstract

Sensitivity-enhanced 2D IPAP experiments using the accordion principle for measuring one-bond 13C'-13Calpha and 1Halpha-13Calpha dipolar couplings in proteins are presented. The resolution of the resulting spectra is identical to that of the decoupled HSQC spectra and the sensitivity of the corresponding 1D acquisitions are only slightly lower than those obtained with 3D HNCO and 3D HN(COCA)HA pulse sequences due to an additional delay 2Delta. For cases of limited resolution in the 2D 15N-1HN HSQC spectrum the current pulse sequences can easily be modified into 3D versions by introducing a poorly digitized third dimension, if so desired. The experiments described here are a valuable addition to the suites available for determination of residual dipolar couplings in biological systems.

摘要

本文介绍了利用手风琴原理进行灵敏度增强的二维IPAP实验，用于测量蛋白质中一键13C'-13Calpha和1Halpha-13Calpha偶极耦合。所得光谱的分辨率与去耦HSQC光谱相同，由于额外的延迟2Delta，相应一维采集的灵敏度仅略低于使用3D HNCO和3D HN(COCA)HA脉冲序列获得的灵敏度。对于二维15N-1HN HSQC光谱分辨率有限的情况，如果需要，可以通过引入数字化程度较差的第三维，轻松将当前脉冲序列修改为三维版本。这里描述的实验是用于确定生物系统中剩余偶极耦合的可用套件中的一个有价值的补充。