在动态轴向压缩模式下，使用软质和半硬质填充材料进行尺寸排阻色谱法。
Size exclusion chromatography on soft and semi-rigid packing materials in the dynamic axial compression mode.
作者信息
Baru M B, Danilov A V, Vagenina I V
机构信息
Branch of Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow Region 142290, Puschino, Russian Federation.
出版信息
J Biochem Biophys Methods. 2003 Aug 29;57(2):115-42. doi: 10.1016/s0165-022x(03)00084-8.
The effect of dynamic axial compression within a range of up to 5 bar upon the structure of the bed packed with soft and semi-rigid packing materials (Sephadex G-25, Bio-Gel P2 and Toyopearl HW-40) and the associated chromatographic parameters were studied for size exclusion chromatography. Continuous packing compression is accomplished by use of a special column with controlled external pressure applied to the packing. Compression has been shown to favor an overall increase in the resolution with pressure optima observed in some cases.
研究了高达5巴压力范围内的动态轴向压缩对填充有软质和半硬质填充材料（葡聚糖凝胶G - 25、生物凝胶P2和东曹HW - 40）的柱床结构及相关尺寸排阻色谱参数的影响。通过使用一种对填充物施加可控外部压力的特殊柱子来实现连续的填充压缩。结果表明，压缩有利于整体分辨率的提高，在某些情况下还观察到了最佳压力。
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