Matrex cellufine GH 25的评估

Evaluation of Matrex cellufine GH 25.

作者信息

Vincent Patrick, Compoint Jean-Pierre, Fitton Valérie, Santarelli Xavier

机构信息

Ecole Supérieure de Technologie des Biomolécules de Bordeaux (ESTBB), UMR 5544, Université Victor Segalen Bordeaux 2, 146 rue Léo Saignat, 33076 Bordeaux Cedex, France.

出版信息

J Biochem Biophys Methods. 2003 Jun 30;56(1-3):69-78. doi: 10.1016/s0165-022x(03)00073-3.

DOI:10.1016/s0165-022x(03)00073-3

PMID:12834969

Abstract

The aim of this work was to test a new matrix for group size exclusion chromatography, Matrex cellufine GH 25, and compare it with Sephadex G25 Superfine and Sephadex G25 fine. Matrex cellufine GH 25 showed a better behaviour at high flow rate (792 cm/h) without back-pressure or packing-down. Sephadex G25 superfine showed a limited flow rate (226 cm/h) with both back-pressure and packing-down and Sephades G25 fine allowed running at 792 cm/h but with packing-down fourfold superior to that of Matrex cellufine GH 25. To have the same number of theoretical plates, it was necessary to increase the height of the bed and therefore the volume of the matrix (43%). With Matrex cellufine GH 25, the sample volume was more limited (9% of column volume) than with Sephadex G25 superfine (17%) but was equivalent to Sephadex fine (9%). To have the same elution time as Sephadex G25 superfine, the flow rate had to be increased by a factor 1.7. As Matrex cellufine GH 25 allowed a high flow rate when the volume of the sample was limited to 6% of column volume, its performance is better than that of Sephadex G25.

摘要

这项工作的目的是测试一种用于尺寸排阻色谱的新型基质Matrex cellufine GH 25，并将其与葡聚糖凝胶G25超细型和葡聚糖凝胶G25精细型进行比较。Matrex cellufine GH 25在高流速（792 cm/h）下表现更佳，没有背压或填料压实现象。葡聚糖凝胶G25超细型的流速有限（226 cm/h），存在背压和填料压实问题，而葡聚糖凝胶G25精细型允许以792 cm/h的流速运行，但填料压实程度是Matrex cellufine GH 25的四倍。为了获得相同数量的理论塔板，有必要增加床层高度，进而增加基质体积（43%）。使用Matrex cellufine GH 25时，样品体积比葡聚糖凝胶G25超细型更受限（占柱体积的9%），但与葡聚糖凝胶G25精细型相当（9%）。为了与葡聚糖凝胶G25超细型有相同的洗脱时间，流速必须提高1.7倍。由于当样品体积限制在柱体积的6%时，Matrex cellufine GH 25允许高流速，其性能优于葡聚糖凝胶G25。