用于腹腔镜检查的不同气体（氦气、二氧化碳、室内空气、氙气）对肿瘤体积、增殖和凋亡的影响。
Influence of different gases used for laparoscopy (helium, carbon dioxide, room air, xenon) on tumor volume, proliferation, and apoptosis.
作者信息
Dähn S, Schwalbach P, Wöhleke F, Benner A, Kuntz C
机构信息
Surgical Department, University of Heidelberg, Im Neuenheimer Feld 110, 69120 Heidelberg, Germany.
出版信息
Surg Endosc. 2003 Oct;17(10):1653-7. doi: 10.1007/s00464-002-9263-2. Epub 2003 Aug 15.
BACKGROUND
Previous reports suggest that helium pneumoperitoneum used for laparoscopic surgery suppresses postoperative tumor growth. The present study was designed to determine the effects of gases used in laparoscopy on tumor volume, proliferation, and apoptosis in rats with implanted malignoma.
METHODS
In 36 rats Morris hepatoma 3294A cells were implanted intrahepatically. Then, after 5 days, they underwent laparoscopy using helium ( n = 7), CO(2) ( n = 7), room air ( n = 7), or xenon ( n = 8). One group received anesthesia only ( n = 7). Rats were killed 10 days after implantation to assess tumor volume, proliferation, and apoptosis.
RESULTS
Helium pneumoperitoneum caused a significant smaller tumor volume compared to other groups (Kruskal-Wallis test: p = 0.001; median tumor volume: control: 44 mm3; helium: 19 mm3). There was no significant difference in tumor cell proliferation (PCNA) and apoptosis (TUNEL reaction) between the groups.
CONCLUSIONS
There was a significant decrease of tumor volume using helium pneumoperitoneum compared to the other gases, but no decreased tumor cell proliferation or increased tumor cell apoptosis.
背景
先前的报告表明，用于腹腔镜手术的氦气气腹可抑制术后肿瘤生长。本研究旨在确定腹腔镜手术中使用的气体对植入恶性肿瘤大鼠的肿瘤体积、增殖和凋亡的影响。
方法
在36只大鼠中，将Morris肝癌3294A细胞植入肝内。然后，5天后，它们接受了使用氦气（n = 7）、二氧化碳（n = 7）、室内空气（n = 7）或氙气（n = 8）的腹腔镜检查。一组仅接受麻醉（n = 7）。植入后10天处死大鼠，以评估肿瘤体积、增殖和凋亡。
结果
与其他组相比，氦气气腹导致的肿瘤体积明显更小（Kruskal-Wallis检验：p = 0.001；肿瘤体积中位数：对照组：44 mm3；氦气组：19 mm3）。各组之间肿瘤细胞增殖（PCNA）和凋亡（TUNEL反应）无显著差异。
结论
与其他气体相比，氦气气腹使肿瘤体积显著减小，但肿瘤细胞增殖未减少，肿瘤细胞凋亡也未增加。
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