[水蚤培养基的化学发光及其测定条件的优化]

[Chemiluminescence of daphnia cultivating medium and optimization of conditions for its determination].

作者信息

Ivashkevich S P, Levkovets I A, Nazarenko V I, Starodub N F

机构信息

Palladin Institute of Biochemistry, NAS of Ukraine, Kyiv.

出版信息

Ukr Biokhim Zh (1999). 2002 May-Jun;74(3):93-7.

PMID:12916243

Abstract

The article deals with the optimization of conditions for the chemiluminescence determination. The Daphnia habitat was shown to have no spontaneous chemiluminescence. This was revealed using hydrogen peroxide and luminol, the optimal concentrations of which were 23 and 1.6 x 10(-2) mmol/L. p-Iodphenol at low concentrations (4 x 10(-5)-2 x 10(-3) mmol/L) did not render its effect chemiluminescence signal while at high concentrations (4 x 10(-2) mmol/L) an inhibition of chemiluminescence was observed. To obtain the needed intensity of chemiluminescence no more than 5 daphnia persons is required to incubate in volume of 10 mL of sample for analyzing. The intensity of chemiluminescence of daphnia cultivating medium and the sensitivity of this organism to potassium chromate increased at the temperature increasing from 24 to 32 degrees C. Daphnia cultivating medium can be preserved in refrigerator for several hours without lost of chemiluminescence signal.

摘要

本文探讨了化学发光测定条件的优化。结果表明，水蚤栖息地不存在自发化学发光现象。通过使用过氧化氢和鲁米诺揭示了这一点，其最佳浓度分别为23和1.6×10⁻² mmol/L。低浓度（4×10⁻⁵ - 2×10⁻³ mmol/L）的对碘苯酚不会对化学发光信号产生影响，而高浓度（4×10⁻² mmol/L）时则会观察到化学发光受到抑制。为获得所需的化学发光强度，分析时在10 mL样品体积中孵育不超过5只水蚤个体即可。随着温度从24℃升高到32℃，水蚤培养基的化学发光强度以及该生物体对铬酸钾的敏感性均增加。水蚤培养基可保存在冰箱中数小时而不会丧失化学发光信号。