Arnhold J, Mueller S, Arnold K, Grimm E
Institute of Biophysics, School of Medicine, University of Leipzig, Germany.
J Biolumin Chemilumin. 1991 Jul-Sep;6(3):189-92. doi: 10.1002/bio.1170060309.
Hydrogen peroxide amplifies the chemiluminescence in the oxidation of luminol by sodium hypochlorite. A linear relationship between concentration of hydrogen peroxide and light intensity was found in the concentration range 5 x 10(-8)-7.5 x 10(-6) mol/l. At 7.5 x 10(-6) mol/l H2O2 the chemiluminescence is amplified 550-fold. The chemiluminescence spectra of these reactions have a wavelength maximum at 431 nm independent of the concentration of hydrogen peroxide. The results indicate that hydrogen peroxide is a necessary component in the chemiluminescent oxidation of the luminol by sodium hypochlorite.
过氧化氢可增强次氯酸钠氧化鲁米诺时的化学发光。在5×10⁻⁸ - 7.5×10⁻⁶ mol/L的浓度范围内，发现过氧化氢浓度与光强度之间呈线性关系。在7.5×10⁻⁶ mol/L的过氧化氢浓度下，化学发光增强了550倍。这些反应的化学发光光谱在431 nm处有最大波长，与过氧化氢浓度无关。结果表明，过氧化氢是次氯酸钠对鲁米诺进行化学发光氧化反应中的必要成分。
