Li Dan-Rong, Hou Hua-Xin, Zhang Wei, Li Li
Clinic Experiment Center, Guangxi Cancer Institute, Nanning, Guangxi, 530021, PR China.
Ai Zheng. 2003 Aug;22(8):801-5.
BACKGROUND & OBJECTIVE: Inducing apoptosis and inhibiting the telomerase activity of tumor cells became a new therapeutic means for tumor. In vivo and in vitro experiments showed that wogonin possesses antioxidant activities and inhibitory effect on tumor cells growth. This study was designed to evaluate the effect of wogonin on telomerase activity and apoptosis of human ovarian carcinoma cell line A2780.
MTT assay,fluorescent microscopy,and DNA agarose gel electrophoresis were used to determine the role of wogonin on apoptosis of A2780 cells. The telomerase activity of A2780 cells were observed by using TRAP-ELASA method.
A2780 cell growth was significantly inhibited by wogonin. The inhibiting effect showed concentration-dependent and time-dependent manners with IC(50) of 85 microg/ml. After treatment with 50 microg/ml and 100 microg/ml wogonin for 48 hours, A2780 cells showed morphological changes associated with the characters of apoptosis under fluorescent microscope. Typical DNA ladder was found using agarose gel electrophoresis. Telomerase activity of A2780 cells was gradually decreased with the increasing of wogonin concentration. When the concentration of wogonin was higher than 200 microg/ml, telomerase activity of A2780 cells was inhibited markedly.
Wogonin can inhibit proliferation and induce apoptosis of A2780 cells within a certain concentration range(50-250 microg/ml). Anticancer effects of wogonin were associated with the induction of apoptosis and partly with the suppression of telomerase activity.
诱导肿瘤细胞凋亡并抑制其端粒酶活性成为肿瘤治疗的新手段。体内外实验表明，汉黄芩素具有抗氧化活性及对肿瘤细胞生长的抑制作用。本研究旨在评价汉黄芩素对人卵巢癌细胞株A2780端粒酶活性及凋亡的影响。
采用MTT法、荧光显微镜及DNA琼脂糖凝胶电泳检测汉黄芩素对A2780细胞凋亡的作用。运用TRAP-ELASA法观察A2780细胞的端粒酶活性。
汉黄芩素显著抑制A2780细胞生长。抑制作用呈浓度和时间依赖性，半数抑制浓度（IC50）为85μg/ml。用50μg/ml和100μg/ml汉黄芩素处理48小时后，荧光显微镜下A2780细胞呈现出与凋亡特征相关的形态学改变。琼脂糖凝胶电泳发现典型的DNA梯状条带。A2780细胞的端粒酶活性随汉黄芩素浓度升高而逐渐降低。当汉黄芩素浓度高于200μg/ml时，A2780细胞的端粒酶活性受到明显抑制。
在一定浓度范围（50 - 250μg/ml）内，汉黄芩素可抑制A2780细胞增殖并诱导其凋亡。汉黄芩素的抗癌作用与诱导凋亡有关，部分与抑制端粒酶活性有关。
